Megazyme O-GM2 61-a-D-半乳岑糖/甘露三糖
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正文中列出的所有试剂只能用于测试或研究，不能作为”药品”，”食品”，”家庭用品”等使用。
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Megazyme代理 K-ACETAF 乙酸[AF法]检测试剂盒
Megazyme代理 K-ACETAF 乙酸[AF法]检测试剂盒
Megazyme试剂盒代理、Megazyme代理
欢迎访问Megazyme官网或者咨询我们获取更多产品信息。
中文名称：乙酸[AF法]检测试剂盒
货号：K-ACETAF
英文名称：Acetic Acid Assay Kit (ACS Analyser Format)
规格：141.6 mL of prepared reagent (e.g. 456 assays of 0.31 mL)
Analyser format UV-method for the determination of Acetic Acid in foodstuffs, beverages and other materials
(乙酰-CoA合成酶)(1)(柠檬酸合成酶)(2)2(L-苹果酸脱氢酶)+170.5 mL 配好的试剂 (R1 + R2)分光光度计，340 nm~15min10mg/L(使用推荐方法)葡萄酒、啤酒、水果和果汁、软饮料、醋、蔬菜、泡菜乳制品（如奶酪）、肉、鱼、面包、焙烤食品（和发酵粉）、番茄酱、酱油、蛋黄酱、调味汁、纸（硬纸板）、茶、医药品（如注射液）、饲料和其他原料（生物培养基、样品等）该实验方法已通过EN、ISO、ICUMSA、德国和荷兰的认证
INTRODUCTION:
The most widely used method for enzymatic quantification of acetic acidis that based on the use of acetyl-coenzyme A synthetase (ACS),according to equations 1 – 3 below. For auto-analyser applications itbecomes necessary to prepare a “master mix” reagent (R1), containing allcomponents of the assay except the reaction initiation enzyme, ACS.However, reagent R1 when prepared from some kits has very limitedon-machine stability, owing to a rapidly increasing absorbance value. Toovercome this issue, Megazyme developed this kit (K-ACETAF), thatdoesn’t exhibit this instability phenomenon. Additionally, theanti-inhibitory compound polyvinylpyrollidone (PVP) has also beenincorporated into the assay to prevent inhibition caused by certaintannins found in grape juice and wine.
试剂盒组成:
Kits suitable for the preparation of 141.6 mL of reagent (equivalent to456 reactions of 0.31 mL) are available from Megazyme.
The kits contain the full assay method plus:
Bottle 1: Buffer (30 mL, pH 8.4) plus L-malic acid, PVP and sodiumazide (0.02 % w/v) as a preservative. Stable for > 2 years at 4°C.
Bottle 2: (x2) NAD+ plus ATP and CoA.Freeze dried powder.Stable for> 5 years at -20°C.
Bottle 3: L-Malate dehydrogenase plus citrate synthase suspension, 2.2mL. Stable for > 2 years at 4°C.
Bottle 4: Acetyl-coenzyme A synthetase suspension (1.1 mL). Stable for> 2 years at 4°C.
REAGENT PREPARATION:
Preparation of R1:
Component Volume
bottle 1 (buffer) 5.50 mL
bottle 2 (NAD+/ATP/CoA) 2.20 mL (after adding 5.50 mL of H2O to bottle 2)
bottle 3 (L-MDH/CS) 0.44 mL (swirl to mix before use)
H2O 18.35 mL
Total volume 26.49 mL
Preparation of R2:
Component Volume
bottle 4 (ACS) 0.22 mL (swirl to mix before use)
H2O 1.60 mL
Total volume 1.82 mL
EXAMPLE METHOD:
R1: 0.290 mL
Sample: ~ 0.005 mL
R2: 0.020 mL
Reaction time: 15 min at either 20-25°C or 37°C
Wavelength: 340 nm
Prepared reagent stability: > 3 days when refrigerated
Calculation: endpoint
Reaction direction: increase
Linearity: up to 30 μg/mL of acetic acid in final reaction solution
Principle原理:
(acetyl-CoA synthetase)
(1) Acetic acid + ATP + CoA → acetyl-CoA + AMP + pyrophosphate
(citrate synthase)
(2) Acetyl-CoA + oxaloacetate + H2O → citrate + CoA
(L-malate dehydrogenase)
(3) L-Malate + NAD+ ↔ oxaloacetate + NADH + H+Kit size: 141.6 mL of prepared reagent (R1 + R2)
Method: Spectrophotometric at 340 nm
Reaction time: ~ 15 min
Detection limit: 10 mg/L (recommended assay format)
Application examples:
Wine, beer, fruit and fruit juices, soft drinks, vinegar, vegetables,
pickles, dairy products (e.g. cheese), meat, fish, bread, bakery products
(and baking agents), ketchup, soy sauce, mayonnaise, dressings,
paper (and cardboard), tea, pharmaceuticals (e.g. infusion solutions),
feed and other materials (e.g. biological cultures, samples, etc.)
Method recognition:
Methods based on this principle have been accepted by EN, ISO,
ICUMSA, IFU and MEBAK
Wine, beer, fruit and fruit juices, soft drinks, vinegar, vegetables,
pickles, dairy products (e.g. cheese), meat, fish, bread, bakery products
(and baking agents), ketchup, soy sauce, mayonnaise, dressings,
paper (and cardboard), tea, pharmaceuticals (e.g. infusion solutions),
feed and other materials (e.g. biological cultures, samples, etc.)
Method recognition:
Methods based on this principle have been accepted by EN, ISO,
ICUMSA, IFU and MEBAK
Advantages
- No wasted ACS solution (stable suspension supplied)
- PVP incorporated to prevent tannin inhibition
- Very stable reagent when prepared for auto-analyser applications (> 3 days at 4°C)
- Linear calibration up to 30 μg/mL of acetic acid in final reaction solution
- Validated by the University of Wine, Suze la Rousse, France
- Very competitive price (cost per mL of reagent)
- All reagents stable for > 2 years after preparation
优点：
不会浪费ACS溶液（提供的 为稳定的悬浮液）加入PVP防止丹宁酸的抑制自动分析检测时配置好的试剂非常稳定 (> 5天， 4摄氏度)
最终反应液中的乙酸线性高达已通过法国葡萄酒大学验证
价格低廉 (每ml试剂的成本)所有试剂配制后的稳定性 >2年
Megazyme S-AZFR5 内切型菊粉酶检测底物 Azo-Fructan 5g
Megazyme S-AZFR5 内切型菊粉酶检测底物 Azo-Fructan 5g
Megazyme试剂盒代理、Megazyme代理
欢迎访问Megazyme官网或者咨询我们获取更多产品信息。
中文名称：内切型菊粉酶检测底物
英文名：Azo-Fructan – 5g
货号：S-AZFR5
规格：5 grams
品牌： Megazyme
High purity dyed, soluble Azo-Fructan for the measurement of enzyme activity, for research, biochemical enzyme assays and in vitro diagnostic analysis.
Substrate for the measurement of endo-inulinase.
Megazyme D-葡萄糖[GOPOD法]检测试剂盒
Megazyme D-葡萄糖[GOPOD法]检测试剂盒
Megazyme试剂盒代理、Megazyme代理
欢迎访问Megazyme官网或者咨询我们获取更多产品信息。
中文名称：D-葡萄糖[GOPOD法]检测试剂盒
英文名：D-Glucose (GOPOD Format) Assay Kit
货号：K-GLUC
规格：660 assays per kit
品牌： Megazyme
分析物意义：常见食品组分，在某些情况下非常重要，如糖尿病产品
Megazyme检测试剂盒优点：选择简单可用的方法，葡萄糖氧化酶/过氧化酶 /己糖激酶/6-磷酸葡萄糖脱氢酶。试剂稳定
The D-Glucose test kit contains high purity reagents for the measurement and analysis of D-glucose in cereal extracts and for use in combination with other Megazyme kits.
Colourimetric method for the determination of D-Glucose in
foodstuffs, beverages and other materials
Principle:
(glucose oxidase)
(1) D-Glucose + H2O + O2 → D-gluconate + H2O2
(peroxidase)
(2) 2H2O2 + p-hydroxybenzoic acid + 4-aminoantipyrine →
quinoneimine + 4H2O
Kit size: 660 assays
Method: Spectrophotometric at 510 nm
Reaction time: ~ 20 min
Detection limit: 100 mg/L
Application examples:
Wine, beer, fruit juices, soft drinks, milk, jam, dietetic foods, bakery
products, candies, fruit and vegetables, tobacco, cosmetics, pharmaceuticals,
feed, paper and other materials (e.g. biological cultures, samples, etc.)
Method recognition:
Widely used and accepted in clinical chemistry and food analysis
Advantages
- All reagents stable for > 12 months after preparation
- Very competitive price (cost per test)
- Simple format
- Standard included
FAQ解答
Q1. There is an issue with the performance of the kit; the results are not as expected.
If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:
- Ensure that you have tested the standard sample that is supplied with the Megazyme test kit.
- Send the results of the kit standard, blank samples and the results obtained for your sample, in the relevant MegaCalc spreadsheet (if available) to Megazyme (cs@megazyme.com). Where available the relevant MegaCalc spreadsheet can be downloaded from where the product appears on the Megazyme website.
- State the kit lot number being used (this is found on the outside of the kit box).
- State which assay format was used (refer to the relevant page in the kit booklet if necessary).
- State exact details of any modifications to the standard procedure that is provided by Megazyme.
- State the sample type and describe the sample preparation steps if applicable.
Q2. I have some questions regarding the Glucose Oxidase and peroxidase in the Glucose Assay Kit.
At what optimal pH do the enzymes work? Does the sample that is added to the enzyme mix show incorrect results if it is outside a certain pH range?
The test is best run at pH 7.4. The reagent is buffered at this pH. Using different pH values will affect results, i.e. it may take longer to reach this end point; but the same end-point value should be obtained if not too far away from this pH value.
Q3. What is the linear range of the Glucose Assay Kit?
The test is set up to measure between 10 and 100 micrograms per assay (i.e. 0.1 mL of 0.1 to 1.0 mg/mL). You may prefer to use 3.0 mL of GOPOD reagent mixture plus 1.0 mL of sample; in this case the concentration range in the material being analysed (diluted) should be ~10 to 100 micrograms per mL. The test is linear up to an absorbance of 1.4 (final assay volume of 3.2 mL). If the final volume is 4.0 mL, then linearity will be up to about 1.0 absorbance units.
Q4. I have some questions on your Glucose Assay Kit. Is the colour stable at room temperature? Does the reaction continue if not read within 20 minutes?
The reaction is complete after approx. 15 min, and the colour is stable at temperatures of 20-50˚C for at least an extra hour.
Q5. Please let us know the lower limit of detection of measuring glucose using your Glucose Assay Kit (mg/mL).
1 microgram gives an absorbance of 0.01 if 3 mL of GOPOD are used. If 1 mL of GOPOD is used, 1 microgram gives an absorbance of 0.03 OD.
Q6. Should the pH of the sample be adjusted even for samples in acidic media?
The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.
Q7. Sometimes a negative absorbance change is obtained for the blank samples, is this normal? Should the real value (negative absorbance change) or “0” be used in the calculation of results?
Sometimes the addition of the last assay component can cause a small negative absorbance change in the blank samples due to a dilution effect and in such cases it is recommended that the real absorbance values be used in the calculation of results.
Q8. We stored the Glucose Assay Kit at 0-5°C for some weeks, because that is what is written on the package. Now we have noticed on some of the vials it says storage at –20°C. Can you tell me how long the products can be stored at 0-5°C without damage?
The kit enzymes can be stored at 0-5°C for up to 12 months, so they will be perfectly fine.
Q9. The temperature for the Glucose Assay Procedure (40 or 50˚C)-is this for incubation of the mix or reading the results at the spectrophotometer? Could I read the results at room temperature?
The temperature is just for the incubations. It is an end-point assay, so the spectrophotometer does not need to be temperature controlled.
Q10. Can oligosaccharides or polysaccharides be measured using the kit assay?
The kit assay will only measure the non-covalently linked monosaccharide.
Oligosaccharides or polysaccharides can be measured after hydrolysis to the monosaccharide. Generally acid hydrolysis can be achieved by boiling the oligo/polysaccharide in 1.3 M HCl for 1 h. It is recommended that scientific literature is consulted for information on hydrolysis conditions for the particular oligo/polysaccharide that is being measured.
Megazyme K-DMAL D-苹果酸检测试剂盒 D-Malic Acid Assay Kit 100U
Megazyme K-DMAL D-苹果酸检测试剂盒 D-Malic Acid Assay Kit
Megazyme试剂盒代理、Megazyme代理
欢迎访问Megazyme官网或者咨询我们获取更多产品信息。
中文名称：D-苹果酸检测试剂盒
英文名：D-Malic Acid Assay Kit
货号：K-DMAL
规格：100 assays (manual) / 1000 assays (micropl
The D-Malic Acid assay kit is suitable for the specific measurement and analysis of D-malic acid (D-malate) in beverages and food products.
Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.
Suitable for manual, auto-analyser and microplate formats.
UV-method for the determination of D-Malic Acid in foodstuffs,
beverages and other materials
原理Principle:
(D-malate dehydrogenase)
(1) D-Malic acid + NAD+ → pyruvate + CO2 + NADH + H+
Kit size: 100 assays (manual) / 1000 (microplate)
/ 1100 (auto-analyser)
Method: Spectrophotometric at 340 nm
Reaction time: ~ 6 min
Detection limit: 0.26 mg/L
Application examples:
Wine, beer, fruit juices, soft drinks, dietetic foods, candies, fruit and
vegetables, cosmetics, pharmaceuticals and other materials
(e.g. biological cultures, samples, etc.)
Method recognition:
Methods based on this principle have been accepted by EEC, EN,
DIN, OIV, IFU, and AIJN
Advantages 优点
- No wasted D-malate dehydrogenase solution (stable suspension supplied)
- Very competitive price (cost per test)
- All reagents stable for > 2 years after preparation
- Rapid reaction (even with difficult samples)
- Mega-Calc™ software tool is available from our website for hassle-free raw data processing
- Standard included
- Extended cofactors stability
- Suitable for manual, microplate and auto-analyser formats
Megazyme K-DMAL D-苹果酸检测试剂盒 D-Malic Acid Assay Kit 其他相关试剂盒
D-苹果酸检测试剂盒 K-DMAL
D-葡萄糖检测试剂盒 K-FRGLMQ
酵母葡聚糖酶检测试剂盒 K-EBHLG
亚硫酸盐总量（酶法）检测试剂盒 K-ETSULPH
L-乳酸快速检测试剂盒 K-DLATE
D-乳酸快速检测试剂盒 K-DATE
柠檬酸检测试剂盒 K-CITR
内切纤维素酶检测试剂盒(CELLG3方法) K-CELLG3
β-淀粉酶检测试剂盒 K-BETA3
β-葡聚糖[混联]检测试剂盒 K-BGLU
乙酸[乙酸激酶法]检测试剂盒 K-ACETRM
阿拉伯聚糖检测试剂盒 K-ARAB
阿斯巴甜检测试剂盒 K-ASPTM
氨（快速）检测试剂盒 K-AMIAR
乙醛检测试剂盒 K-ACHYD
天冬酰胺快速检测试剂盒 K-ASNAM
谷氨酰胺和氨快速检测试剂盒 K-ASNAM
L-阿拉伯糖检测试剂盒 K-ARGA
D-半乳糖检测试剂盒 K-ARGA
α-淀粉酶检测试剂盒 K-CERA
乙酸检测试剂盒 K-ACET
木聚糖酶(Azo-Wax)检测试剂盒 K-AZOWAX
直链淀粉检测试剂盒 K-AMYL
支链淀粉检测试剂盒 K-AMYL
乙酸GK检测试剂盒（分析仪格式） K-ACETGK
乙酸【AK法】检测试剂盒 K-ACETAK
维生素C（L-抗坏血酸）检测试剂盒 K-ASCO
可吸收碳水化合物检测试剂盒 K-ACHDF
膳食纤维检测试剂盒 K-ACHDF
α-葡萄糖醛酸酶检测试剂盒 K-AGLUA
乙酸[AF法]检测试剂盒 K-ACETAF
β-葡聚糖检测试剂盒（酵母和蘑菇） K-YBGL
亚硫酸盐总量检测试剂盒 K-TSULPH
D-木糖检测试剂盒 K-XYLOSE
淀粉总量HK检测试剂盒 K-TSHK
海藻糖检测试剂盒 K-TREH
D-半乳糖醛酸检测试剂盒 K-URONIC
D-葡萄糖醛酸检测试剂盒 K-URONIC
木聚糖酶检测试剂盒 K-XYLS
尿素[快速]检测试剂盒 K-URAMR
氨[快速]检测试剂盒 K-URAMR
膳食纤维总量质控粉套装 K-TDFC
淀粉总量(AA/AMG)检测试剂盒 K-TSTA
淀粉总量质控粉套装 K-TSCK
总膳食纤维检测试剂盒 K-TDFR
蔗糖检测试剂盒 K-SUCGL
抗性淀粉检测试剂盒 K-RSTAR
游离亚硫酸盐总量检测试剂盒 K-SULPH
琥珀酸检测试剂盒 K-SUCC
丁二酸检测试剂盒 K-SUCC
棉子糖检测试剂盒 K-RAFGA
蜜三糖检测试剂盒 K-RAFGL
棉子糖检测试剂盒 K-RAFGL
蔗糖检测试剂盒 K-RAFGL
D-半乳糖检测试剂盒 K-RAFGL
蔗糖检测试剂盒 K-SUFRG
破损淀粉检测试剂盒 K-SDAM
D-果糖检测试剂盒 K-SUFRG
D-葡萄糖检测试剂盒 K-SUFRG
酒石酸检测试剂盒 K-TART
D-葡萄糖检测试剂盒 K-SUCGL
山梨醇检测试剂盒 K-SORB
木糖醇检测试剂盒 K-SORB
L-鼠李糖检测试剂盒 K-RHAMNOSE
抗性淀粉控粉套装 K-RSTCL
丙酮酸检测试剂盒 K-PYRUV
果胶鉴定试剂盒 K-PECID
D-半乳糖检测试剂盒 K-RAFGA
植酸检测试剂盒 K-PHYT
L-精氨酸检测试剂盒 K-LARGE
乳糖检测试剂盒 K-LACGAR
乳糖检测试剂盒 K-LACSU
尿素检测试剂盒 K-LARGE
D-甘露糖检测试剂盒 K-MANGL
D-果糖检测试剂盒 K-MANGL
D-葡萄糖检测试剂盒 K-LACSU
乳糖检测试剂盒 K-LACSU
麦芽糖检测试剂盒 K-MASUG
蔗糖检测试剂盒 K-MASUG
D-甘露醇检测试剂盒 K-MANOL
L-阿拉伯糖醇检测试剂盒 K-MANOL
D-葡萄糖检测试剂盒 K-MANGL
D-葡萄糖检测试剂盒 K-MASUG
乳果糖检测试剂盒 K-LACTUL
D-半乳糖检测试剂盒 K-LACGAR
氨[快速]检测试剂盒 K-LARGE
D-葡萄糖检测试剂盒 K-LACSU
综合总膳食纤维检测试剂盒 K-INTDF
肌醇检测试剂盒 K-INOSL
L-谷氨酸检测试剂盒 K-GLUT
D-3羟丁酸检测试剂盒 K-HDBA
葡甘露聚糖检测试剂盒 K-GLUM
D-葡萄糖[HK法]检测试剂盒 K-GLUHKR/L
D-葡萄糖检测试剂盒 K-GLUC
D-果糖检测试剂盒 K-FRGLMQ
D-果糖检测试剂盒 K-FRGLQR
D-葡萄糖酸检测试剂盒 K-GATE
D-果糖检测试剂盒 K-FRUGL
D-葡萄糖检测试剂盒 K-FRUGL
蚁酸检测试剂盒 K-FORM
葡萄糖氧化酶检测试剂盒 K-GLOX
半乳甘露聚糖检测试剂盒 K-GALM
D-葡萄糖检测试剂盒[液体即用型] K-FRGLQR
乙醇检测试剂盒 K-ETOH
D-葡萄糖胺检测试剂盒 K-GAMINE
L-谷氨酸酰胺快速）检测试剂盒 K-GLNAM
氨（快速）检测试剂盒 K-GLNAM
D-葡萄糖酸内脂检测试剂盒 K-GATE
甘油检测试剂盒 K-GCROL
果聚糖（HK法）测试剂盒 K-FRUCHK
甘油GK法检测试剂盒 K-GCROLGK
L-岩藻糖检测试剂盒 K-FUCOSE
Sigma 孕酮Progesterone
Sigma 孕酮Progesterone
产品编号 P8783 | CAS号 57-83-0 | SIGMA
英文名:Progesterone
中文名:1.孕甾酮 2.黄体酮 3.黄体素 4.孕酮 5.孕醇 6.黄体激素
Synonym: 4-Pregnene-3,20-dione
CAS Number 57-83-0
Empirical Formula (Hill Notation) C21H30O2
Molecular Weight 314.46
description:synthesized from materials derived from plant source
product line:BioReagent
form:powder
potency:0.1-20 ng/mL
mp:128-132 °C(lit.)
solubility :absolute ethanol: soluble1 mg/mL
suitability :uitable for cell culture
Application
用于细胞培养，研究细胞信号传导和基因表达的甾醇调控
Other Notes
由卵巢黄体产生的类固醇激素。
包装：1, 5, 25 g in glass bottle
Reconstitution
To prepare 20μg/ml stock solution, add 1ml absolute ethanol per mg progesterone, gently swirl to dissolve, add 49ml sterile medium per ml ethanol.
Physical form
室温下为粉末状；分成小份冻存，以避免使用时反复冷冻
Biochem/physiol Actions
诱导子宫内皮细胞的成熟和分泌活动;抑制排卵。孕酮与
Sigma人血管紧张素 II
Sigma人血管紧张素 II
产品编号 A9525 | CAS号 4474-91-3 | SIGMA
Angiotensin II human ≥93% (HPLC), powder
| CAS号 4474-91-3 分子式: C50H71N13O12 分子量 1046.18 assay ≥93% (HPLC) form powder total impurities 1.2-10.4% acetate storage temp. -20°C Amino Acid Sequence Asp-Arg-Val-Tyr-Ile-His-Pro-Phe Antibody Adsorbant Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-OH · 2CH3COOH · H2O Analysis Note 肽含量不低于 75% Biochem/physiol Actions 血管紧张素 (Ang) II 在调节心血管的血液动力学和结构中起重要作用。Ang II 在成人组织内的大部分已知效应均可归因于血管紧张素 II 类型 I (AT1) 受体。AT1 和 AT2 受体在药理学和生物化学性质上有差别，在心血管血液动力学和细胞生长方面表现出相反的效应。此外，肾素-血管紧张素和一氧化氮生成系统在心血管功能的调节中显示出相互作用的现象。Ang II 刺激血管生成，增加微血管密度。事实上，血管紧张素 II 是由血管紧张素转换酶对血管紧张素原的作用而生成；C-端-组氨酰-亮氨酸裂解。 |
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| 保存条件： | -20°C |
Sigma丝裂霉素 C 来源于头状链霉菌
Sigma丝裂霉素 C 来源于头状链霉菌
产品编号 M0503-2mg | CAS号 50-07-7 | SIGMA
Frequently Asked Questions
Frequently Asked Questions
are available for this Product.
Application
Mitomycin C is produced by a strain of actinomyces, Streptomyces caespitosus. It contains three anticancer moieties, quinine, urethane, and aziridine groups. It is used to generate mitotically inactive feeder cells in cell culture systems, such as the mitotically inactive fibroblasts used in embryonic stem cell systems.
Biochem/physiol Actions
DNA 合成、细胞核分裂和癌细胞的抑制剂。对革兰氏阳性菌、
Caution
This vial contains 2 mg Mitomycin C and 48 mg NaCl. Stock solutions should be filter sterilized and stored at 2-8°C in the dark. Solutions at pH 6-9 can be stored at 0-5°C for up to a week, but if a precipitate forms, a fresh solution should be prepared – the precipitated solution has been proven toxic to cells.
Preparation Note
Mitomycin C is soluble in water at .5 mg/mL, with a pH of 6.0-7.5. It undergoes rapid degradation in acidic solutions with pH<6, and is mostly likely to retain activity in solutions with a pH between 6-9.
Protocols & Applications
Antibiotic Selector for application, solubility, solution stability, working concentration, and mode of action information
Sigma 阿霉素 盐酸盐
Sigma 阿霉素 盐酸盐
产品编号 D1515 | CAS号 25316-40-9 | SIGMA
Frequently Asked Questions
Frequently Asked Questions are available for this Product.
包装:10 mg in glass bottle Biochem/physiol Actions
Naturally fluorescent anthracycline antibiotic, anticancer drug. Doxorubicin is a substrate of MRP1 which was first cloned from a DOX-resistant lung cancer cell line. Fluorescent property has been exploited for the measurement of drug efflux pump activities as well as resolving the important question of intracellular localization of various multidrug resistance proteins and the role of subcellular organelles (Golgi and lysosome) in the sequestration of drugs and its implication in drug resistant phenotypes.
法律信息
Adriamycin 为以下机构的注册商标： RDF Pharmacia & Upjohn S.P.A.
Protocols & Applications
| Related Categories | Antibiotics, Antibiotics A to Z, Antibiotics A-F, Antibiotics by Application, Antineoplastic and Immunosuppressive Antibiotics, |
| assay | 98.0-102.0% (HPLC) |
| mp | 216 °C (dec.)(lit.) |
| solubility | H2O: soluble50 mg/mL, clear |
| Mode of action | DNA synthesis | interferes |
| antibiotic activity spectrum | viruses |
| storage temp. | 2-8°C |
Sigma 放线菌素 D
Sigma 放线菌素 D
产品编号 A9415 | CAS号 50-76-0 | SIGMA
Frequently Asked Questions
Frequently Asked Questions are available for this Product.
Application
Actinomycin D is an antineoplastic antibiotic that inhibits cell proliferation with wide-ranging applications ranging from a selection agent in cell culture, use in studies of suppressing HIV-replication and programmed cell death of PC12 cells, and as an antibiotic in treatment of various malignant neoplasms, including Wilm′s tumour and the sarcomas. Recommended for us in cell culture applications at 1μg/mL.
Caution
This product is hygroscopic and light-sensitive. If stored protected from light and moisture at 2-8°C, it remains unchanged, by HPLC testing, for at least 15 months. Unused dilute solutions of Actinomycin D are extremely sensitive to light and tend to adsorb to plastic and glass and should be discarded. However, frozen aliquots of concentrated stock solution are stable for at least a month.
包装：2, 5, 10, 25 mg in glass bottle
Preparation Note
Actinomycin D is sold as red, shiny crystals and is soluble in acetonitrilie or acetone at 10 mg/mL and in DMSO at a minimum of 1 mg/mL. Some references also show slight solubility in water of 0.5 mg/mL.
Biochem/physiol Actions
Actinomycin D inhibits the proliferation of cells by forming a stable complex with double-stranded DNA, inhibiting DNA-primed RNA synthesis and causing single-stranded breaks in DNA. It has been shown to be an inhibitor of the minus-strand transfer step in reverse transcriptase.
Protocols & Applications