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爱尔兰Megazyme 产品清单现货表

爱尔兰Megazyme是生产分析试剂、酶和食品酶法试剂盒的公司，是开发饮料、谷类食品、乳制品、食品、饲料、发酵、生物燃料和葡萄酒行业提供试剂盒和试剂的公司。从公司成立之初的检测试剂盒试剂盒生产，到现在新的领域，碳水化合物的和谷物酶相关行业发展已成为检测领域领导者之一。

爱尔兰Megazyme 产品清单现货表

B-BISTRIS250	Bis-Tris Buffer salt 250 grams
B-CAPS200	CAPS Buffer salt 200 grams
B-CAPSO250	CAPSO Buffer salt 250 grams
B-GLYGLY250	GLYCYLGLYCINE BUFFER salt
B-HEPES250	HEPES Buffer salt 250 grams
B-MES250	MES monohydrate Buffer salt
B-MOPS250	MOPS Buffer salt 250 grams
B-PIPES250	PIPES Buffer salt 250 grams
B-TRIS500	Tris Buffer salt 500 grams
C-ATP100	Adenosine 5′-triophosphate (ATP)
C-ATP20	Adenosine 5′-triphosphate (ATP) 20g
C-CLFR10	Calcofluor Fluorescent Stain
C-CLFR5	Calcofluor Fluorescent Stain
C-COA500	Coenzyme A (trilithium salt) 500mg
C-NAD25	ß-NicotinamideAdenine Di-nucleotide
C-NAD5	ß-NicotinamideAdenine Di-nucleotide
C-NADH10	ß-Nicotinamide Adenine
C-NADH2	ß-Nicotinamide Adenine
C-NADP10	ß-Nicotinamide adenine
C-NADP2	ß-Nicotinamide adenine
D-ADFTB	Advanced Dietary Fibre Technology
D-AMYL	Amylose / Amylopetin DVD
D-BETA3	ß-Amylase(BETAMYL 3 method) DVD
D-BGLU	ß-Glucan (Mixed Linkage) DVD
D-CERA	Ceralpha: Alpha Amylase
D-FRGLMQ	Megaquant Meter+
D-IBMKIII	Megazyme Incubation Bath MKIII
D-INTDF	Integrated Total Dietary Fibre
D-INTDFB	Water Bath for Integrated Total
D-LMALMQ	Megaquant Meter+L-Malic Acid
D-MBGL	Malt ß-Glucanse DVD
D-MQTUB	Tubes for the Megaquant Colorimeter
D-PRET	Sample Pre-treatment Format
D-SDAM	Starch Damage DVD
D-TSTA	Total Starch DVD
D-WINE	Wine Testing Assay Format DVD
E-ABFCJ	Alpha-L-Arabinofuranosidase
E-ABFCT	Alpha-L-Arabinofuranosidase
E-ACKEC	Acetate Kinase -Libios Only
E-ACPEC	Acid phosphatase (E.coli) 400U
E-ACSBS	Acetyl-CoA synthase (B. subtilis)
E-ADHEC	Alcohol Dehyrogenase (E.coli)
E-AFAM2	Alpha-L-Arabinofuranosidase
E-AFASE	Alpha-L-Arabinofuranosidase
E-AGLAN	Alpha-Galactosidase (A. niger)
E-AGLGU	Alpha-Galactosidase (Guar) 1000U
E-AGUBS	a-D-Glucuronidase
E-ALGLS	Alginate lyase (Sphingomonas sp)
E-ALPEC	Alkaline phosphatase (E.coli) 400U
E-AMGDF	Amyloglucosidase (A.niger)
E-AMGDF10	Amyloglucosidase (10mL)
E-AMGDF100	Amyloglucosidase (A.niger) 100ml
E-AMGDF20	Amyloglucosidase (20mL)
E-AMGFR100	Amyloglcosidase (A.niger) 100mg
E-AMGFR500	Amyloglucosidase (A.niger) 500mg
E-AMGPU	Amyloglucosidase (Rhizopus sp.) 5KU
E-AMPK	Adenylate Kinase (myokinase)
E-ANAAM	Alpha-Amylase (A. oryzae) 20KU
E-ANAGM	Alpha-N-acetylgalactosylamindase
E-APKLDH	Acetate kinase/ Pyruvate kinase /
E-ARBACJ	endo-/exo-Arabinanase (C.japonicus)
E-ASNEC	Asparaginase (E.coli) 400U
E-AXEAO	Acetylxylan esterase (Orpinomyces
E-AXEAOB	Acetylxylan esterase (Orpinomyces
E-BAASS	Alpha-Amylase
E-BAMBC	ß-Amylase (B.cereus) 20K units
E-BARBL	ß-Amylase (Barley; liquid) 100KU
E-BARBP	ß-Amylase (Barley; powder)
E-BGLAEC	ß-Glucuronidase (E.coli)
E-BGLAN	ß-Galactosidase (A.niger) 8KU
E-BGLUC	ß-Glucosidase (A.niger) 200U
E-BGOSAG	ß-Glucosidase – 600U.
E-BGOSTM	ß-Glucosidase
E-BLAAM	Alpha-Amylase (B. licheniformis)
E-BLAAM100	Alpha-Amylase (B.licheniformis)
E-BMABS	endo-1,4-ß-Mannanase (bacillus sp.)
E-BMACJ	endo-1,4-ß-Mannanase (C.japonicus)
E-BMANN	endo-1,4-ß-Mannanase (A.niger) 500U
E-BMATM	endo-1,4-ß- Mannanase (T.maritima)
E-BMOSCF	ß-Mannosidase (C. fimi) – 200U
E-BNAHEX	ß-N-acetylhexosaminidase
E-BSPRPD	Protease (Subtilisin A)
E-BSPRT	Protease (Subtilisin A)
E-BSPRT10	Protease (Subtilisin A)
E-BSPRT100	Protease (Subtilisin A) (100mL)
E-BSPRT20	Protease (subtilisin A) 20mL
E-BXSEBP	ß-Xylosidase (B.pumilus) 200U
E-BXSR	exo-1,4-ß-D-Xylosidase
E-BXSRB	exo-1,4-ß-D-Xylosidase
E-CBHI	Cellobiohydrolase I – 20mg
E-CELAN	Cellulase (A. niger) – 2,000U
E-CELBA	Cellulase (B. amyloliquefaciens)
E-CELTE	Cellulase (T.emersonii) 2,000 U
E-CELTM	Cellulase (T.maritima) 2K units
E-CELTR	Cellulase (endo-1,4-ß-D-glucanase)
E-CITEC	Citrate synthase (E. coli) 2500U
E-CMPK	Cytidylate Kinase (prokaryote)
E-CREA	Creatinase
E-DIAEC	Diaphorase (E. coli) 1000U
E-DIPEP	Alpha-aspartyl dipeptidase (E.coli)
E-DLDHLM	D-Lactate dehydrogenase
E-DMDHEC	D-Malate Dehydrogenase (E. coli)
E-EARAB	endo-1,5-alpha-L-Arabinanase 400U
E-EGALN	endo-1,4-B-D-Galactanase 1KU
E-ENDOI	endo-Inulinanase (A.niger) 200U
E-EXBGL	exo-1,3-ß-D-Glucanase 200U
E-EXBGOS	exo-1,3-ß-D-glucanase 300 units +
E-EXBGTV	exo-1,3-Beta-glucanase (GH5) 400u
E-EXG5AO	exo-1,3-Beta-glucanase(GH5) 500u
E-EXOI	exo-Inulinase (A.niger) ( 5,000U )
E-EXOIAN	exo-Inulinase (A.niger) (GH32)
E-FAERU	Feruloyl Esterase (Rumen
E-FAEZCT	Feruloyl Esterase
E-FDHCB	Formate dehydrogenase (C.boidinii)
E-FRMXLQ	Fructanase Mixture (Purified) 20ml
E-FUCTM	Alpha Fucosidase (T.maritima)
E-GALCJ	endo-1,4-ß-D-Galactanase
E-GALCT	endo-1,4-ß-D-Galactanase
E-GALDH	Galactose Dehydrogenase
E-GALMUT	Galactose Dehydrogenase /
E-GAMP	Glucoamylase P (Hormoconis resinae)
E-GLUKEC	Gluconokinase (E.coli)
E-GLUTEC	Glutaminase (E.coli) 2500U
E-GMPK	Guanylate Kinase (prokaryote)
E-GOTEC	Glutamate Oxaloacetate Transaminase
E-GOXCA	Glucose Oxidase / Catalase Mixture
E-GPDH5	Glucose 6-Phosphate Dehydrogenase
E-GPDHEC	Glucose 6-phosphate dehydrogenase
E-GPO	Glycerol 3-Phosphate Oxidase
E-GPTBS	Glutamate pyruvate transaminase
E-HBDH	3-Hydroxybutyrate dehydrogenase
E-HEX10	Hexokinase from Yeast 10,000U
E-HKGDH	Hexokinase (420 U/ml) +
E-HYLSP	Hyaluronate lyase 50U
E-ICDHBS	Isocitrate dehydrogenase
E-INDHBS	myo-Inositol Dehydrogenase
E-INVPD2	Invertase Powder 2 grams
E-INVPD5	Invertase Powder 5 grams
E-INVRT	Invertase (B-Fructofuranosidase)
E-ISAMY	Isoamylase 1000U
E-LAMHV	endo-1,3-ß-D-Glucanase (Barley)
E-LAMSE	endo-1,3-ß-D-Glucanase 100U
E-LICACT	Non specific Endo 1,3(4)ß-glucanase
E-LICHN	Lichenase(endo-1,3(4)-ß-Dglucanase)
E-LLDHP	L-Lactate dehyrogenase (Porcine)
E-LMDHEC	L-Malate dehydrogenase 50,000U
E-MALTS	Alpha-Glucosidase (Maltase)
E-MAST	Malt Amylase Standard 100 mL
E-MNHPF	Mannitol dehydrogenase
E-NADHPO	NADH Peroxidase (E. faecalis)
E-OAGUM	Oligo-1,6-Glucosidase (GH13)
E-OGLYEF	Endo-a-N-acetylgalactosaminidase
E-PANAA	Alpha Amylase (Porcine Pancreas)
E-PCLYAN	Pectate Lyase in 50% Glycerol
E-PCLYAN2	Pectate Lyase (Aspergillus sp.)
E-PGALS	endo-Polygalacturonanase M1
E-PGALUSP	endo-Polygalacturonanase M2
E-PGDHEC	6-Phosphogluconate dehydrogenase
E-PGIBS	Phosphoglucose Isomerase
E-PGIBSB	Phosphoglucose Isomerase
E-PGIEC	Phosphoglucose Isomerase
E-PGIECB	Phosphoglucose Isomerase
E-PGISC	Phosphoglucose Isomerase
E-PGISCB	Phosphoglucose Isomerase
E-PGM	Alpha-Phosphoglucomutase(microbial)
E-PLYCJ	Pectate Lyase (C. japonicus) 2500U
E-PMIEC	Phosphomannose Isomerase
E-PTABS	Phosphotransacetylase (B.subtilis)
E-PULBL	Pullulanase M2 (B. licheniformis)
E-PULKP	Pullulanase M1 (K. planticola)
E-RHAMS	Alpha-Rhamnosidase (prokaryote)
E-SCOAS	Succinyl-CoA synthetase
E-SIALCP	Exo-Alpha-Sialidase (C.perfringens)
E-SIALST	Exo-Alpha-Sialidase (S.typhimurium)
E-SUCR	Sucrase (Maltase) (yeast) 300 U
E-SUCRBG	Sucrase plus ß-Galactosidase
E-TMPK	Thymidylate Kinase (prokaryote)
E-TREH	Trehalase (prokaryote) 4,200 units
E-TRNGL	Alpha-Glucosidase(Transglucosidase)
E-TSAGL	Alpha-Glucosidase 1,500 U
E-TSAGS	Alpha-Glucosidase
E-UAO	Uricase (eukaryote)- 300 units
E-XANLB	Xanthan Lyase (Bacillus sp)
E-XEGP	Xyloglucanase (Paenibacillus sp)
E-XGP74	Xyloglucanase (GH74)
E-XYAN4	Xylanase M4 (A.niger) 8000 U
E-XYLATM	endo-1,4-ß-Xylanase (T.maritima)
E-XYLNP	endo-1,4-ß-Xylanase (N.patriciarum)
E-XYNACJ	endo-1,4-ß-Xylanase (C.japonicus)
E-XYNAP	endo-1,4-ß-Xylanase (A.punctata)
E-XYNBCM	endo-1,4-ß-Xylanase (C.mixtus)
E-XYNBS	endo-1,4-ß-Xylanase (B.stearother
E-XYRU6	Xylanase M6 (rumen microorganism)
E-XYTR1	Xylanase M1 (T.viride) 8000 U
E-XYTR2	Xylanase M2 (T.longibrachiatum;
E-XYTR3	Xylanase M3 (T.longibrachiatum;
G-AMBH	Ambersep 200 H+ Ion Exchange Resin
G-AMBOH	Amberlite FPA OH Ion Exchange
G-CEL100	Celite – 100g
G-CEL500	Celite – 500g
G-LCYST200	L-Cysteine Hydrochloride
I-AAVIC	Azo-Avicel 10g
I-ACELL	Azo-Alpha-Cellulose 10g
I-AZAMY	AZCL-Amylose 5g
I-AZBGL	AZCL-Barley ß-Glucan 3g
I-AZCAS	AZCL-Casein 4g
I-AZCEL	AZCL-HE-Cellulose 3g
I-AZCHAN	AZCL Chitosan 4 grams
I-AZCOL	AZCL-Collagen 4g
I-AZCUR	AZCL-Curdlan 3g
I-AZDAR	AZCL-Arabinan (Debranched) 3g
I-AZDEX	AZCL-Dextran (No.B512) 3g
I-AZGLP	AZCL-Galactan (Potato) 3g
I-AZGMA	AZCL-Galactomannan (carob) 3g
I-AZPAC	AZCL-Pachyman 3g
I-AZPUL	AZCL-Pullulan 3g
I-AZRHI	AZCL-Rhamnogalacturonan I – 2grams
I-AZWAX	AZCL-Arabinoxylan (Wheat) 3g
I-AZXBW	AZCL-Xylan (Birchwood) 4g
I-AZXYG	AZCL-Xyloglucan (Tamarind) 4g
K-ACET	Acetic Acid (ACS; manual format)
K-ACETAF	Acetic Acid (ACS; analyser format)
K-ACETAK	Acetic Acid (AK; analyser format)
K-ACETRM	Acetic Acid (AK; rapid manual
K-ACHDF	Available Carbohydrates/Dietary
K-ACHYD	Acetaldehyde
K-AGLUA	Alpha-Glucuronidase
K-AMIAR	Ammonia (Rapid)
K-AMYL	Amylose/Amylopectin
K-ARAB	Arabinan
K-ASCO	L-Ascorbic Acid (L-Ascorbate)
K-ASNAM	L-Asparagine/L-Glutamine/ Ammonia
K-ASPGN	Asparagine/Aspartic Acid
K-ASPTM	Aspartame
K-AZOWAX	Xylanase (Azo-Wax Format)
K-BETA3	ß-Amylase (Betamyl-3 method)
K-BGLU	ß-Glucan
K-CERA	Alpha Amylase (Ceralpha method)
K-CITR	Citric Acid
K-DATE	D-Lactic Acid (D-Lactate) (Rapid)
K-DLATE	D/L-Lactic Acid
K-DMAL	D-Malic Acid
K-EBHLG	ß-Glucan (Yeast-Enzymatic)
K-ETOH	Ethanol
K-ETSULPH	Total Sulphite Kit (Enzymatic)
K-FORM	Formic Acid
K-FRGLMQ	D-Fructose/D-Glucose (MegaQuant
K-FRGLQR	D-Fructose/ D-Glucose (Liquid Ready
K-FRUC	Fructan
K-FRUCHK	Fructan (Hexokinase format)
K-FRUGL	D-Fructose / D-Glucose
K-FUCOSE	L-Fucose Kit
K-GALM	Galactomannan (Carob or guar)
K-GAMINE	D-Glucosamine (D-Glucosamine
K-GATE	D-Gluconate/D-Glucono-d-lactone
K-GCROL	Glycerol
K-GCROLGK	Glycerol GK
K-GLNAM	L-Glutamine/Ammonia (Rapid)
K-GLOX	Glucose Oxidase
K-GLUC	D-Glucose (GOPOD format)
K-GLUHKL	D-Glucose (Hexokinase format;large)
K-GLUHKR	D-Glucose(Hexokinase format;regular
K-GLUM	Glucomannan
K-GLUT	L-Glutamic Acid (MSG)
K-HDBA	D-3-Hydroxybutyric Acid
K-INTDF	Integrated Total Dietary Fibre
K-ISOC	D-Isocitric Acid
K-LACGAR	Lactose / D-Galactose (Rapid)
K-LACSU	Lactose/Sucrose/D-Glucose
K-LACTUL	Lactulose
K-LARGE	L-Arginine/Urea/Ammonia (Rapid)
K-LATE	L-Lactic Acid
K-LMALAF	L-Malic Acid (Analyser format)
K-LMALL	L-Malic Acid
K-LMALMQ	L-Malic Acid (MegaQuant format)
K-LMALQR	L-Malic Acid (Liquid Ready Reagent)
K-LMALR	L-Malic Acid (Regular)
K-MALTA	Malt Amylase
K-MANGL	D-Mannose/D-Fructose/D-Glucose
K-MANOL	D-Mannitol / L-Arabitol
K-MASUG	Maltose/Sucrose/D-Glucose
K-MBGL	ß-Glucanase (Malt & microbial)
K-PANOPA	Primary Amino Nitrogen (PANOPA)
K-PECID	Pectin Identification
K-PHYT	Phytic Acid/Total Phosphorus
K-PYRUV	Pyruvic Acid
K-RAFGA	Raffinose/D-Galactose
K-RAFGL	Raffinose/Sucrose/D-Glucose
K-RHAM	L-Rhamnose
K-RSTAR	Resistant Starch
K-RSTCL	Resistant Starch Control Flours
K-SDAM	Starch Damage
K-SORB	D-Sorbitol / Xylitol
K-SUCC	Succinic Acid
K-SUCGL	Sucrose / D-Glucose
K-SUFRG	Sucrose/ D-Fructose/ D-Glucose
K-SULPH	Total and Free Sulphite
K-TART	Tartaric Acid
K-TDFC	Total Dietary Fibre Controls
K-TDFR	Total Dietary Fibre
K-TREH	Trehalose
K-TSCK	Total Starch Control Flours
K-TSTA	Total Starch (AA/AMG)
K-TSULPH	Total Sulphite
K-URAMR	Urea/Ammonia (Rapid)
K-URONIC	D-Glucuronic/D-Galacturonic
K-XYLOSE	D-Xylose
K-XYLS	Xylanase (Xylazyme AX format)
K-YBGL	ß-Glucan (Yeast & Mushroom)
L-CONA1000	Concanavalin A (Con A) 1g
L-CONA200	Concanavalin A (Con A) – 200mg
O-4MUXBI-10	4-Methylumbelliferyl-ß-Xylobioside
O-4MUXBI-5	4-Methylumbelliferyl-ß-Xylobioside
O-ABI	Arabinobiose (syrup) – 50mg
O-AHE	Arabinohexaose (powder) – 20mg
O-AHP	Arabinoheptaose (powder) – 15mg
O-AMX	Aldouronic Acid Mixture 200mg
O-AMXR	Aldouronic Acid Mixture (Reduced)
O-AOC	Arabino-Octaose (powder) – 10mg
O-APE	Arabinopentaose (syrup) – 30mg
O-ATE	Arabinotetraose (syrup) – 30mg
O-ATR	Arabinotriose (syrup) – 50mg
O-BGTETA	1,3:1,4 ß-Glucotetraose (A) 10mg
O-BGTETB	1,3:1,4 ß-Glucotetraose (B) 40mg
O-BGTETC	1,3:1,4 ß-Glucotetraose (C) 30mg
O-BGTRIA	1,3:1,4 ß-Glucotriose (A) 50mg
O-BGTRIB	1,3:1,4 ß-Glucotriose (B) 50mg
O-BNPG4	Blocked BNPG – 4 Vials
O-CHE	Cellohexaose – 20mg
O-CHERD	1,4-ß-D-Cellohexaitol (borohydride
O-CHI2	Diacetyl-Chiotobiose – 30mg
O-CHI3	Triacetyl-Chitotriose 30mg
O-CHI4	Tetraacetyl-Chitotetraose – 20mg
O-CHI5	Pentacetyl-Chitopentaose- 20mg
O-CHI6	Hexaacetyl-Chitohexaose- 10mg
O-CPE	Cellopentaose – 30mg
O-CPERD	1,4-ß-D-Cellopentaitol (borohydride
O-CTE	Cellotetraose – 50mg
O-CTERD	1,4-ß-D-Cellotetraitol (borohydride
O-CTR	Cellotriose – 50mg
O-CTRRD	1,4-ß-D-Cellotriitol (borohydride
O-GBI	Galactobiose – 50mg
O-GGM5	63,64-A-D- Galactosyl-Mannopentaose
O-GM3	61-A-D-Galactosyl-Mannotriose -20mg
O-GMH	A-D-Glucosyl-Maltotriosyl-Maltriose
O-GMM3	Galactosyl Mannobiose+Mannotriose
O-GMMBI	1,4-ß-D-Glucosyl-D-Mannose plus
O-GMMTR	1,4-ß-D-Glucosyl-D-Mannobiose and
O-GMT	63-A-D-Glucosyl-Maltotriose – 50mg
O-IPRM	Isoprimeverose (xyloglucan derived)
O-KPE	1,1,1-Kestopentaose – 40mg
O-KTE	1,1-Kestotetraose – 100mg
O-KTR	1-Kestose – 100mg
O-LAM2	Laminaribiose – 50 mg
O-LAM3	Laminaritriose – 50 mg
O-LAM4	Laminaritetraose – 40 mg
O-LAM5	Laminaripentose – 30 mg
O-LAM6	Laminarihexaose – 30 mg
O-MBI	Mannobiose – 50mg
O-MHE	Mannohexaose – 20mg
O-MPE	Mannopentaose – 30mg
O-MTE	Mannotetraose – 30mg
O-MTR	Mannotriose – 50mg
O-ONPXBI-25	ortho-Nitrophenyl-ß-xylobioside
O-ONPXBI-5	ortho-Nitrophenyl-ß-xylobioside
O-PNPXBI-10	p-Nitrophenyl-ß-xylobiose 10mg
O-PNPXBI-50	p-Nitrophenyl-ß-xylobiose 50mg
O-VER	Verbascose – 50mg
O-X3G4	Heptasaccharide (X3 Glc 4) – 50mg
O-X3G4R	Heptasaccharide Reduced
O-XBI	Xylobiose – 50mg
O-XCBIR	Xylosyl Cellobiose (reduced) 50mg
O-XGHDP	HigherDP Xyloglucan Oligosaccharide
O-XGHON	Xyloglucan (Hepta+Octa+Nona
O-XHE	Xylohexaose – 10mg
O-XPE	Xylopentaose – 10mg
O-XTE	Xylotetraose – 30mg
O-XTR	Xylotriose – 50mg
OC-BNPG4	Blocked PNP Maltoheptaoside – 1Vial
P-ARAB	Arabinan (Sugar Beet) – 8g
P-ARGAL	Arabinogalactan (Larch Wood) 10 G
P-BGBH	ß-Glucan (Barley;High Viscosity) 5g
P-BGBL	ß-Glucan (Barley;Low Viscosity) 5g
P-BGBM	ß-Glucan (Barley;Med Viscosity) 5g
P-BGCFA	ß-Glucan CFA Standard – 4Vials
P-BGOH	ß-Glucan (Oat; High Viscosity) 5g
P-BGOM	ß-Glucan (Oat; Med Viscosity) 5g
P-BGYST	ß-Glucan (Yeast, Alkali Soluble) 2g
P-BLDX	ß-Limit Dextrin – 10g
P-BLDX50	ß-Limit Dextrin 50 grams
P-CMC4M	Carboxymethyl Cellulose 4M (CMC-4M)
P-CMCUR	CM-Curdlan 4g
P-CMLA	CM-Linear-1,5-A-L-Arabinan – 2Vial
P-CMPAC	CM-Pachyman – 4g
P-CURDL	Curdlan 8g
P-DBAR	Debranched Arabinan (sugar beet) 2g
P-GALLU	Galactan (Lupin) 3g
P-GALMH	Galactomannan Carob (High Vis) 5g
P-GALML	Galactomannan Carob (Low Vis) 4g
P-GALPOT	Galactan (Potato) 3g
P-GGM21	Galactomannan (guar; galactose
P-GGM28	Galactomannan (guar; galactose
P-GGMHV	Guar Galactomannan (High Vis) 4g
P-GGMMV	Guar Galactomannan (Med Vis) 4g
P-GLCMH	Glucomannan (Konjac:High Vis) 3gr
P-GLCML	Glucomannan (Konjac;Low Vis) 4g
P-LARB	Linear 1,5-A-L-Arabinan – 500mg
P-LICHN	Lichenan (Icelandic Moss) 4g
P-MANCB	Mannan (1,4-B-D-Mannan) 4gr
P-MANIV	Mannan (Ivory Nut) 3g
P-MWBGS	ß-Glucan MW Standard 6x500mg
P-PACHY	Pachyman (1,3-ß-D-Glucan) 5g
P-PGACT	Polygalacturonic Acid (PGA) 10g
P-PGALU	Pectic Galactan (Lupin) 4g
P-PGAPT	Pectic Galactan (Potato) 4g
P-PULLBH	Pullulan (NaBH4 Reduced) 5g
P-PULLN	Pullulan 10g
P-RAXY	Arabinoxylan- Rye Flour 3g
P-RHAGN	Rhamnogalacturonan (soy bean) 5g
P-RHAM1	Rhamnogalacturonan I (Potato)
P-WAXYH	Arabinoxylan (High Vis) -3g
P-WAXYI	Arabinoxylan (Wheat Fl. Insol) 5gr
P-WAXYL	Arabinoxylan (Wheat Fl:Low Vis) 3gr
P-WAXYM	Arabinoxylan (Med Vis) – 3g
P-XYGLN	Xyloglucan (Tamarind) 3g
R-AMGR3	Amyloglucosidase Assay Reagent – 4V
R-AMHR4	Amylase HR Reagent – 4 vials
R-BAMR3	Betamyl 3; ß-Amylase Assay Reagent
R-CAAR4	Ceralpha; A-Amylase Reagent- 4Vials
R-GLC4	Glucose Determination Reagent 4Vial
S-ABG100	Azo-Barley Glucan 100ml
S-ACGLM	Azo-Carob Galactomannan 4g
S-ACMC	Azo-CM-Cellulose (powder) 4g
S-ACMCL	Azo-CM-Cellulose (liquid) 100ml
S-AGALP	Azo-Galactan (Potato) 4g
S-AWAXL	Azo-Wheat Arabinoxylan (liquid)
S-AWAXP	Azo-Wheat Arabinoxylan (powder) 3g
S-AXBL	Azo-Xylan (Birchwood)(liquid) 100ml
S-AXBP	Azo-Xylan (Birchwood)(powder) 3g
S-AXYO	Azo-Xylan (Oat Spelt) 3g
S-AZCAS	Azo-Casein (Sulphanilamide Dyed)
S-AZFR5	Azo-Fructan – 5gr
S-AZFRXOI	Azo-Fructan+exo-Inulinanase 2g
S-AZRH	AZ-Rhamnogalacturonan 2g
S-AZXG	Azo-Xyloglucan (Tamarind) 4g
S-RDAR	Red Debranched Arabinan 2g
S-RPUL	Red Pullulan 3g
S-RSTAR	Red Starch – 5gr
T-AMZ1000	Amylazyme – 1000 Tablets
T-AMZ200	Amylazyme – 200 Tablets
T-AMZBG200	Amylazyme BG – 200 Tablets
T-AMZRD1000	Amylazyme Red – 1000 Tablets
T-AMZRD200	Amylazyme Red – 200 Tablets
T-ARZ200	Arabinazyme – 200 Tablets
T-BGZ1000	Beta-Glucazyme – 1000 Tablets
T-BGZ100MG	Beta-Glucazyme (100mg) 1000 Tablets
T-BGZ200	Beta-Glucazyme – 200 Tablets
T-CCZ1000	Cellazyme C – 1000 Tablets
T-CCZ200	Cellazyme C – 200 Tablets
T-CHZ200	Chitozyme – 200 Tablets
T-CTZ1000	Cellazyme T – 1000 Tablets
T-CTZ200	Cellazyme T – 200 Tablets
T-CUR200	1,3-Beta-Glucazyme HS – 200 Tablets
T-DEXT200	Alpha-Dextrazyme – 200 Tablets
T-GLZ1000	Galactazyme – 1000 Tablets
T-GLZ200	Galactazyme – 200 Tablets
T-LDZ1000	Limit-Dextrizyme – 1000 Tablets
T-LDZ200	Limit-Dextrizyme – 200 Tablets
T-MNZ1000	Mannazyme – 1000 Tablets
T-MNZ200	Mannazyme – 200 Tablets
T-PAZ200	1,3-Beta-Glucazyme 200 Tabs
T-PRAK1000	Protazyme AK – 1000 Tablets
T-PRAK200	Protazyme AK – 200 Tablets
T-PROL1000	Protazyme OL – 1000 Tabs
T-PROL200	Protazyme OL – 200 Tabs
T-PSYL200	Psyllazyme – 200 Tablets
T-RHAM200	Rhamnozyme Tablets – 200 tabs
T-XAX1000	Xylazyme AX (60mg) 1000 Tablets
T-XAX200	Xylazyme AX – (60mg) 200 Tablets
T-XTH200	Xanthazyme Tablets – 200 Tablets
T-XYZ1000	Xylazyme (100mg) – 1000 Tablets
T-XYZ200	Xylazyme (100mg) – 200 Tablets
T-XYZATF	Xylazyme ATF (40mg) 1000 tablets

库存信息随时变更，准确货期以下单时确认为准。

图片仅供参考,请以实物为准。
正文中列出的所有试剂只能用于测试或研究，不能作为”药品”，”食品”，”家庭用品”等使用。
我司所销售的化学试剂、原料等所有产品（包括但不限于抗生素类、蛋白质类、试剂盒类产品等）仅限用于科学研究用途，不得作用于人体。

Megazyme官网及其产品分类

在生命科学领域，上海金畔生物为业界提供了丰富的实验室和生物医药生产研发的产品。在这里要感谢广大客户多年来对上海金畔生物科技有限公司的支持和厚爱，我们将一如既往的为广大客户带来Megazyme高品质的产品和服务，欢迎广大新老客户来电咨询。

Megazyme官网：

Megazyme代理，Megazyme试剂盒代理，Megazyme酶代理

爱尔兰Megazyme是一家制造和供应高品质和创新的检测谷物、食品、饲料、发酵、乳制品和葡萄酒行业的技术。Megazyme成立于1988年，该公司目前提供了超过70种试剂盒和超过300种其他试剂和底物。

该公司产品分以下系列：

1. 生物及食品酶法分析试剂盒 (Diagnostic Kits)
2. 试剂混合物(Reagent Mixtures)
3. 协同因子(Cofactors)
4. 糖酶片剂试验(Carbohydrase Tablet Tests)
5. 蛋白酶片剂试验(Protease Test Tablets)
6. 可溶性呈色底物(Soluble Chromogenic Substrates)
7. 不可溶性（交联的）呈色底物(Insoluble (Crosslinked) Chromogenic Substrates)
8. 酶(Enzymes)
9. 植物凝血素(Lectins)
10. 多聚糖(Polysaccharides)
11. 低聚糖(Oligosaccharides)
12. 相关产品 (General)

Megazyme公司简介：

Megazyme公司位于美丽的爱尔兰，是全球知名的酶及酶法分析试剂盒供应商，他们的很多产品都被AOAC(Association of Official Analytical Chemists)、(European Brewing Convention)、RACI (Royal Australian Chemical Institute) 等国际组织采用，Megazyme公司分析试剂盒广泛应用于食品、饲料、乳品、发酵工业、葡萄糖生产及成品控制，其中欧洲检测果汁及葡萄酒等发酵产品基本都采用Megazyme公司的产品。

Megazyme酶法分析检测原理

NADH（(或者 NADPH）在340nm波长处具有强吸收峰，Megazyme酶法分析检测产品利用这一原理，选择消耗或者产生NADH（(或者 NADPH）的化学反应，利用分光光度计检测NADH（(或者NADPH）浓度的变化，从而达到检测待检物质浓度的目的。

Megazyme酶法分析检测系列包括：

1. 生物及食品酶法分析试剂盒 (Diagnostic Kits)

麦芽淀粉酶检测试剂盒 K-MALTA

酸类检测试剂盒

醇类检测试剂盒

醛类检测试剂盒

氨类检测试剂盒

氮类检测试剂盒

盐/酯类检测试剂盒

酶类检测试剂盒

糖类检测试剂盒

淀粉类检测试剂盒

其中产品被国际机构采用，建立国际检测方法如下：

总淀粉检测盒（Total Starch Assay Kit）
• AOAC Method 996.11
• AACC Method 76-13
• ICC Standard Method no. 168
• RACI Standard Method
总膳食纤维检测盒（Total Dietary Fibre Assay Kit）
• AOAC Method 985.29
• AOAC Method 991.42
• AOAC Method 993.19
• AOAC Method 991.43
• AACC Method 32.07
• AACC Method 32.21
• AACC Method 32.05
淀粉损失检测盒（Starch Damage Assay Kit）
• AACC Method 76-31
• ICC Standard No. 164
• RACI Standard Method
α-淀粉酶检测盒Ceralpha (α-Amylase) Assay Kit
•    AOAC Method 2002.01
•    ICC Standard No. 303
•    RACI Standard Method
•    CCFRA Flour Testing Working Group Method 0018
•    AACC – under evaluation.
Alpha淀粉酶检测盒Amylazyme (α-Amylase Assay)
• AACC Method 22.05
• RACI Standard Method
果聚糖检测盒Fructan Assay Kit
• AOAC Method 999.03
• AACC Method 32-32
木聚糖酶AX片剂（Xylazyme AX Tablets）
• Xylazyme AX Tablets have been widely adopted in the fermentation and feeds industries for the measurement of Xylanase.
β-葡聚糖检测盒 β-Glucan (Mixed Linkage) Assay Kit
• AACC Method 32-23
• EBC Methods 3.11.1, 4.16.1 and 8.11.1
• AOAC Method 995.16
• ICC Standard No. 166
• RACI Standard Method
极限糊精酶和支链淀粉酶检测片剂（Limit-Dextrizyme）
• RACI Standard Method
β-葡聚糖酶检测盒（β-Glucanase (Malt and Microbial) Assay Kit）
• RACI Standard Method
β-淀粉酶检测盒（Betamyl (β-Amylase Assay Kit)）
• RACI Standard Method.
木聚糖酶检测底物（Azo-Wheat Arabinoxylan ）
• Azo-Wheat Arabinoxylan, Azo-CM-Cellulose and Ceralpha methods for the assay of Xylanase, cellulase and ?-Amylase, respectively, have been adopted by the UK silage industry.
抗性淀粉检测盒（Resistant Starch Assay Kit）
• AOAC Method 2002.02

温馨提示：不可用于临床治疗。

2.试剂混合物

3.蛋白酶检测片剂

4.糖酶片剂

5.可溶性呈色底物

6.植物凝血素

7.纤维类检测试剂盒

8.胶质类检测试剂盒

9.不可溶性（交联）呈色底物

10.酶制剂

11.协同因子

12.多糖/聚糖/多聚糖

我们常年提供以下试剂盒：

麦芽淀粉酶检测试剂盒 K-MALTA

乙酸检测试剂盒货号： K-ACET

乙酸[AF法]检测试剂盒货号：K-ACETAF

乙酸【AK法】检测试剂盒货号： K-ACETAK

乙酸GK检测试剂盒货号：（分析仪格式） K-ACETGK

乙酸[乙酸激酶法]检测试剂盒货号： K-ACETRM

可吸收碳水化合物/膳食纤维检测试剂盒货号：K-ACHDF

乙醛检测试剂盒货号：K-ACHYD

α-葡萄糖醛酸酶检测试剂盒货号：K-AGLUA

氨（快速）检测试剂盒货号：K-AMIAR

直链淀粉/支链淀粉检测试剂盒货号： K-AMYL

阿拉伯聚糖检测试剂盒货号：K-ARAB

L-阿拉伯糖/D-半乳糖检测试剂盒货号：K-ARGA

维生素C（L-抗坏血酸）检测试剂盒货号：K-ASCO

天冬酰胺/谷氨酰胺和氨快速检测试剂盒货号：K-ASNAM

阿斯巴甜检测试剂盒货号：K-ASPTM

木聚糖酶(Azo-Wax)检测试剂盒货号：K-AZOWAX

β-淀粉酶检测试剂盒货号：K-BETA3

β-葡聚糖[混联]检测试剂盒货号：K-BGLU

内切纤维素酶检测试剂盒货号：(CELLG3方法) K-CELLG3

α-淀粉酶检测试剂盒货号：K-CERA

柠檬酸检测试剂盒货号：K-CITR

D-乳酸快速检测试剂盒货号：K-DATE

D-/L-乳酸快速检测试剂盒货号：K-DLATE

D-苹果酸检测试剂盒货号：K-DMAL

酵母葡聚糖酶检测试剂盒货号：K-EBHLG

乙醇检测试剂盒货号：K-ETOH

亚硫酸盐总量（酶法）检测试剂盒货号：K-ETSULPH

蚁酸检测试剂盒货号： K-FORM

D-果糖/D-葡萄糖（MegaQuant格式）检测试剂盒货号：K-FRGLMQ

D-果糖/D-葡萄糖检测试剂盒货号：[液体即用型] K-FRGLQR

果聚糖（HK法）测试剂盒货号： K-FRUCHK
D-果糖/D-葡萄糖检测试剂盒货号： K-FRUGL

L-岩藻糖检测试剂盒货号：K-FUCOSE

半乳甘露聚糖检测试剂盒货号：K-GALM

D-葡萄糖胺检测试剂盒货号： K-GAMINE

D-葡萄糖酸 / D-葡萄糖酸内脂检测试剂盒货号：K-GATE

甘油检测试剂盒货号： K-GCROL

甘油GK法检测试剂盒货号： K-GCROLGK

L-谷氨酸酰胺/氨（快速）检测试剂盒货号： K-GLNAM

葡萄糖氧化酶检测试剂盒货号： K-GLOX

D-葡萄糖（GOPOD格式）检测试剂盒货号： K-GLUC

D-葡萄糖[HK法]检测试剂盒货号： K-GLUHKR/L

葡甘露聚糖检测试剂盒货号：K-GLUM

L-谷氨酸检测试剂盒货号：K-GLUT

D-3羟丁酸检测试剂盒货号：K-HDBA

肌醇检测试剂盒货号： K-INOSL

D-异柠檬酸检测试剂盒货号：K-ISOC

乳糖/D-半乳糖检测试剂盒货号：K-LACGAR

乳糖/蔗糖/D-葡萄糖检测试剂盒货号：K-LACSU

乳果糖检测试剂盒货号：K-LACTUL

L-精氨酸/尿素/氨[快速]检测试剂盒货号：K-LARGE

L-乳酸检测试剂盒货号：K-LATE

L-苹果酸（分析仪格式）检测试剂盒货号：K-LMALAF

L-苹果酸检测试剂盒货号： K-LMALL

L-苹果酸（MegaQuant）检测试剂盒货号：K-LMALMQ

L-苹果酸检测试剂盒货号：（液体即用型）K-LMALQR

L-苹果酸检测试剂盒货号：K-LMALR

麦芽淀粉酶检测试剂盒货号： K-MALTA

D-甘露糖/D-果糖/D-葡萄糖检测试剂盒货号：K-MANGL

D-甘露醇/L-阿拉伯糖醇检测试剂盒货号：K-MANOL

麦芽糖/蔗糖/D-葡萄糖检测试剂盒货号：K-MASUG

β-葡聚糖酶(麦芽和微生物)检测试剂盒货号：K-MBGL

初级氨基氮（NOPA）检测试剂盒货号：K-PANOPA

果胶鉴定试剂盒货号：K-PECID

植酸检测试剂盒货号： K-PHYT
丙酮酸检测试剂盒货号：K-PYRUV

棉子糖/D-半乳糖检测试剂盒货号：K-RAFGA

蜜三糖/棉子糖/蔗糖/D-半乳糖检测试剂盒货号：K-RAFGL

L-鼠李糖检测试剂盒货号： K-RHAMNOSE

抗性淀粉检测试剂盒货号：K-RSTAR

抗性淀粉控粉套装K- RSTCL

破损淀粉检测试剂盒货号：K-SDAM

山梨醇/木糖醇检测试剂盒货号：K-SORB

琥珀酸/丁二酸检测试剂盒货号：K-SUCC

蔗糖/D-葡萄糖检测试剂盒货号：K-SUCGL

蔗糖/D-果糖/D-葡萄糖检测试剂盒货号：K-SUFRG

游离亚硫酸盐总量检测试剂盒货号：K-SULPH

酒石酸检测试剂盒货号：K-TART

膳食纤维总量质控粉套装K-TDFC

总膳食纤维检测试剂盒货号： K-TDFR

海藻糖检测试剂盒货号： K-TREH

淀粉总量质控粉套装K-TSCK

淀粉总量HK检测试剂盒货号： K-TSHK

淀粉总量(AA/AMG)检测试剂盒货号：K-TSTA

亚硫酸盐总量检测试剂盒货号：K-TSULPH

尿素/氨[快速]检测试剂盒货号：K-URAMR

D-葡萄糖醛酸和D-半乳糖醛酸检测试剂盒货号：K-URONIC

D-木糖检测试剂盒货号：K-XYLOSE

木聚糖酶检测试剂盒货号：K-XYLS

β-葡聚糖检测试剂盒货号：（酵母和蘑菇) K-YBGL

Megazyme淀粉酶 Amylase

Megazyme淀粉酶 Amylase

上海金畔生物代理Megazyme品牌试剂产品，欢迎新老客户访问Megazyme官网或者咨询我们获取更多相关产品信息。

Megazyme酶试剂分类

淀粉酶

阿拉伯树胶酸

阿拉伯呋喃

纤维二糖水解

纤维素酶

酯酶

果糖酶＆果糖苷酶

岩藻糖苷酶

半乳聚糖酶

半乳糖苷酶

葡聚糖酶

葡萄糖苷酶

葡糖醛酸糖苷

已糖胺酶类

裂解酶

甘露聚糖酶

甘露糖苷酶

支链淀粉酶

唾液酸酶

木聚糖酶

糖苷酶

木葡聚糖酶

多聚半乳糖醛酸(内切)酶

其它酶

Beta淀粉酶[蜡样芽胞杆菌]

英文名：Beta-Amylase (B.cereus) 20K units

货号：E-BAMBC

规格：20000 Units

High purity recombinant β-Amylase (Bacillus cereus) for use in research, biochemical enzyme assays and in vitrodiagnostic analysis.

EC 3.2.1.2
CAZy Family: GH14
CAS: 9000-91-3

beta-amylase; 4-alpha-D-glucan maltohydrolase

Recombinant. From Bacillus cereus.
In 3.2 M ammonium sulphate.

Specific activity: ~ 2,400 U/mg (40^oC, pH 6.5 on soluble starch).

Stability: > 2 years at 4^oC.

1,3-b-D葡聚糖内切酶[木霉菌]

英文名：endo-1,3-β-D-Glucanase (Trichoderma sp.)

货号：E-CELTH

规格：

High purity recombinant Cellulase (endo-1,4-β-D-glucanase) (Alkali Stable) (Thermobifida halotolerans) for use in research, biochemical enzyme assays andin vitro diagnostic analysis.

EC 3.2.1.4
CAZy Family: GH6
CAS: 9012-54-8

1,4-beta-D-glucan 4-glucanohydrolase

Recombinant. From Thermobifida halotolerans.
In 3.2 M ammonium sulphate.
Supplied at ~ 150 U/mL.

Specific activity: ~ 27 U/mg protein (on CM-Cellulose 4M) at pH 8.5 and 60^oC;
~ 16 U/mg protein (on CM-Cellulose 4M) at pH 8.5 and 40^oC.

Stability: > 4 years at 4^oC.

Alpha淀粉酶[米麴菌]

英文名：α-Amylase (Aspergillus oryzae)

货号：E-ANAAM

规格：20000 Units

High purity alpha-Amylase (A. oryzae) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.1
CAZy Family: GH13

From Aspergillus oryzae. Electrophoretically homogeneous.
In 3.2 M ammonium sulphate.

Specific activity: ~ 126 U/mg

Stable at 4^oC for > 4 years. As used in Megazyme Starch Damage method.

Alpha淀粉酶[苔藓杆菌]

英文名：Alpha-Amylase (B.licheniformis)

货号：E-BLAAM-100ML

规格：100mL – 3000 units/mL

High purity alpha-Amylase (B. licheniformis) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.1
CAZy Family: GH13

Alpha-Amylase. From Bacillus licheniformis. Highly purified. For use in Megazyme Total Starch and Dietary Fiber methods. Stabilised solution.

Specific activity: 55 U/mg (40^oC, pH 6.0, Ceralpha reagent as substrate).

Stable at 4^oC for > 4 years.

Data booklets for each pack size are located in the Technical Resources tab.

Alpha淀粉酶[苔藓杆菌]

英文名：Alpha-Amylase (B. licheniformis)

货号：E-BLAAM-10ML

规格：10mL – 3000 units/mL

Alpha淀粉酶[苔藓杆菌]

英文名：Alpha-Amylase (B. licheniformis)

货号：E-BLAAM-40ML

规格：40mL – 3000 units/mL

Alpha淀粉酶[猪胰腺]

英文名：α-Amylase (Porcine Pancreatic)

货号：E-PANAA-3G

规格：3g

High purity alpha-Amylase (Porcine Pancreatic) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.1
CAZy Family: GH13

From porcine pancreas. Partially purified. Free flowing powder.

Specific activity: ca. 100,000 U/g (40^oC, Ceralpha reagent).

Stable at -20^oC for > 4 years.

For use in total dietary fiber (including resistant starch) assay procedure.

Alpha淀粉酶[猪胰腺]

英文名： α-Amylase (Porcine Pancreatic)

货号：E-PANAA-9G

规格：9g

Beta淀粉酶[大麦]

英文名：Beta-Amylase (Barley; liquid) 100KU

货号：E-BARBL-100KU

规格：100000 Units

High purity beta-Amylase (Barley) liquid enzyme for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
EC 3.2.1.2
CAZy Family: GH14
From barley flour. Crystalline.
In 3.2 M ammonium sulphate.
Specific activity: ca. 620 U/mg (40oC, pH 6.0, soluble starch as substrate).
Stable at 4oC for > 4 years.
Powder enzyme form (2000°L) is for use in AACC and ASBC α-amylase assay procedures (see E-BARBP).
Data booklets for each pack size are located in the Technical Resources tab.

Beta淀粉酶[大麦]

英文名：Beta-Amylase (Barley; liquid) 50KU

货号：E-BARBL-50KU

规格：50000 units

Beta淀粉酶[大麦]

英文名：Beta-Amylase (Barley; powder)

货号：E-BARBP-2G

规格：2 grams

Beta淀粉酶[大麦]

英文名：Beta-Amylase (Barley; powder)

货号：E-BARBP-5G

规格：5 grams

α-淀粉酶（解淀粉芽孢杆菌）

英文名：α-Amylase (Bacillus amyloliquefaciens)

货号：E-BAASS

规格：2900 Units

High purity α-Amylase (Bacillus amyloliquefaciens) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.1
CAZY Family: GH13
CAS: 9000-90-2

alpha-amylase; 4-alpha-D-glucan glucanohydrolase

From Bacillus amyloliquefaciens.
In 50% (v/v) glycerol plus 0.02% sodium azide.
Supplied at ~ 140 U/mL.

Specific activity: ~ 50 U/mg (40^oC, pH 6.5 on Ceralpha Reagent).

Stability: > 4 years at -20^oC.

淀粉葡萄糖苷酶

英文名：Amyloglucosidase (Aspergillus niger)

货号：E-AMGFR-100MG

规格：100mg

High purity Amyloglucosidase (A. niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.3
CAZy Family: GH15

From Aspergillus niger. Lyophilised powder. Electrophoretically homogenous.

High purity. Recommended for use in AOAC Method 997.08 (fructan).

Specific activity: ~ 35 U/mg (40^oC, pH 4.5, soluble starch as substrate).

Stable at -20^oC for > 4 years.

Data booklets for each pack size are located in the Technical Resources tab

淀粉葡萄糖苷酶

英文名：Amyloglucosidase (Aspergillus niger)

货号：E-AMGFR-500MG

规格：500mg

淀粉葡萄糖苷酶（黑曲霉）粉末

英文名：Amyloglucosidase (Aspergillus niger) Powder

货号：E-AMGDFPD

规格：4 g – 36000 Units/g

High purity Amyloglucosidase (A. niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.3
CAZY Family: GH15
Amyloglucosidase. From A. niger. High purity. For use in Megazyme Total Starch and Dietary Fiber methods. Specifically prepared for use in AOAC Method 2001.03 which requires enzymes free of glycerol. This AMG is used in combination with thermostable α-amylase (Cat. No. E-BLAAM) and protease powder (Cat. No. E-BSPRTPD). For use, the AMG is dissolved in water at a rate of 1 g/10 mL and 0.2 mL is used per assay. Electrophoretically homogeneous. Lyophilised powder.

Specific activity: approx. 36 U/mg (40°C, pH 4.5, soluble starch as substrate). 3,100 U/g on p-nitrophenyl ß-maltoside; 36,000 U/g on soluble starch.
Stable at -20°C for > 4 years.

淀粉葡萄糖苷酶（黑曲霉）游离甘油

英文名：Amyloglucosidase (Aspergillus niger) Glycerol Free

货号：E-AMGDFNG

规格：50 mL

High purity Amyloglucosidase (Aspergillus niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.3
CAZy Family: GH15
CAS: 9032-08-0

glucan 1,4-alpha-glucosidase; 4-alpha-D-glucan glucohydrolase; glucoamylase

From Aspergillus niger.
In buffered solution.

This enzyme is recommended for use in Total Dietary Fibre analytical procedures and the Megazyme Total Starch test method.

The preparation is free of glycerol so can be used in TDF procedures where glycerol is used as an internal standard (e.g. AOAC Method 2001.03/AACC Method 32-41.01; the Matsutani Method).

Specific activity: ~ 36 U/mg (40^oC, pH 4.5 on soluble starch); ~ 400 U/mL on p-nitrophenyl β-maltoside; ~ 6,520 U/mL on soluble starch.

Stability: > 4 years 4^oC.

淀粉葡萄糖苷酶[根霉]

英文名：Amyloglucosidase (Rhizopus sp.)

货号：E-AMGPU

规格：5000u

High purity Amyloglucosidase (Rhizopus sp.) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.3
CAZy Family: GH15

From Rhizopus sp. Ultra high purity. Electrophoretically homogeneous. Freeze-dried powder.

Specific activity: approx. 35 U/mg (40^oC, pH 4.5, soluble starch as substrate).

Stable for > 4 years at -20^oC.

淀粉葡萄糖苷酶[黑曲霉]

英文名：Amyloglucosidase (Rhizopus sp.)

货号：E-AMGPU

规格：5000u

High purity Amyloglucosidase (Rhizopus sp.) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.3
CAZy Family: GH15

From Rhizopus sp. Ultra high purity. Electrophoretically homogeneous. Freeze-dried powder.

Specific activity: approx. 35 U/mg (40^oC, pH 4.5, soluble starch as substrate).

Stable for > 4 years at -20^oC.

淀粉葡萄糖苷酶[黑曲霉]

英文名：Amyloglucosidase (Aspergillus niger)

货号：E-AMGDF-100ML

规格：326000

High purity Amyloglucosidase (A. niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.3
CAZy Family: GH15

Amyloglucosidase.

From A. niger. High purity. For use in Megazyme Total Starch and Dietary Fiber methods. Electrophoretically homogeneous. Stabilised liquid in 50% glycerol.

Specific activity: approx. 36 U/mg (40^oC, pH 4.5, soluble starch as substrate). 200 U/ml on p-nitrophenyl β-maltoside; 3,260 U/ml on soluble starch.

Stable at 4^oC for > 4 years.

Data booklets for each pack size are located in the Technical Resources tab.

葡聚糖1,4-α-麦芽六糖

英文名：Glucan 1,4-α-maltohexaosidase

货号：E-MAL6M

规格：2000 Units

High purity glucan 1,4-α-maltohexaosidase (microbial) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.98
CAZy Family: GH13
CAS: 72561-12-7

glucan 1,4-alpha-maltohexaosidase; 4-alpha-D-glucan maltohexaohydrolase

Recombinant. From Bacillus species.
In 3.2 M Ammonium sulphate.
Supplied at ~ 500 U/mL.

Specific activity: ~ 800 U/mg protein (on soluble starch) at pH 8.5 and 40^oC.

Stability: > 4 years at 4^oC.

糖化酶P（H. resinae）（重组）

英文名：Glucoamylase P (H. resinae) (Recombinant)

货号：E-GAMP

规格：340 Units at 40oC; ~ 800 Units at 60oC

High purity recombinant Amyloglucosidase (Glucoamylase P – H. Resinae) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.3
CAZy Family: GH15
amyloglucosidase; exo-1,4-alpha-glucosidase; glucan 1,4-alpha-glucosidase

Recombinant from Hormoconis resinae.
In 3.2 M ammonium sulphate.
Hydrolysis of terminal non-reducing α-1,4-D-glycosidic bonds in α-1,4-D-glucans with “debranching activity” (hydrolysis of α-1,6-D-glycosidic bonds) in substrates such as starch and pullulan.
Supplied at ~ 170 U/mL.

Specific activity: ~ 60 U/mg (40^oC, pH 4.5 on soluble starch); ~ 160 U/mg (60^oC, pH 4.5 on soluble starch).
Stability: > 4 years at 4^oC.

异淀粉酶[糖原6-葡萄糖苷酶]

英文名：Isoamylase (Glycogen 6-glucanohydrolase)

货号：E-ISAMY

规格：600 Units

High purity Isoamylase (Glycogen 6-glucanohydrolase) for use in research, biochemical enzyme assays and in vitrodiagnostic analysis.

EC 3.2.1.68
CAZy Family: GH13

From Pseudomonas sp. Electrophoretically homogeneous.
In 3.2 M ammonium sulphate.

Specific activity: 280 U/mg (40^oC, pH 4.0, oyster glycogen) (equivalent to 3 MU Sigma Units/mg).

Stable at 4^oC for > 4 years.

异淀粉酶的HP（糖原果胶6-葡聚糖水解酶）

英文名： Isoamylase HP (Glycogen 6-glucanohydrolase)

货号：E-ISAMYHP

规格：200 Units

英文名： Isoamylase HP (Glycogen 6-glucanohydrolase)

货号：E-ISAMYHP

规格：200 Units

纤维素酶检测试剂盒

纤维素酶检测试剂盒[CellG5法], Cellulase Assay Kit (CellG5 Method)

货号：K-CellG5

中文品名：纤维素酶检测试剂盒[CellG5法]

品名：Cellulase Assay Kit (CellG5 Method)

规格1：K-CellG5-4V 120/240次(手工)/480次(自动分析仪)
规格2：K-CellG5-2V 60/120次(手工)/240次(自动分析仪)

说明：CellG5检测试剂盒用于检测纤维素内切酶（1,4 -β-葡聚糖内切酶）包含两种组分：1) 4,6-O-(3-Ketobutylidene)-4-nitrophenyl-β-D-cellopentaoside (BPNPG5) 和 2)热稳定的β-葡萄糖苷酶。酮阻断基团可阻止β-葡萄糖苷酶对BPNPG5的水解。通过纤维素内切酶的培养可生成非阻断的显色寡糖，被β-葡萄糖苷酶迅速水解。产生4-硝基苯酚的速率与β-葡萄糖苷酶水解BPNPG5直接相关。加入Tris缓冲液(pH 9.0)后可终止反应，并形成苯酚显色产物。
CellG5检测法代表了代表了纤维素酶测量方法的巨大进步。传统的纤维素酶检测依赖于酶底物，例如羧甲基纤维素，微晶纤维素，纤维低聚糖，CMC滤纸，染色多糖，包括CMC刚果红或纤维素天青。

应用：纤维素酶检测试剂盒[CellG5法]。

原理：

比色法测定酶制剂、发酵产品中1,4 -β-葡聚糖内切酶（纤维素酶）的含量。

检测方法：分光光度法 @400 nm

反应时间：10min

检测限：3.5 x 10^-4 U/mL

应用案例：

发酵液，工业酶制剂和生物燃料研究。

方法识别：

新方法

试剂盒组成：

Bottle 1: (x2) 或 (x4)	每瓶含有4,6-O-(3-Ketobutylidene)-4-nitrophenyl-β-D-cellopentaoside (BPNPG5)溶于3毫升含有0.02% w/v叠氮钠的10% DMSO水溶液。
-20°C下稳定超过4年。
Bottle 2: (x 2)	热稳定的β-葡萄糖苷酶(0.50 mL, 600 U/mL)溶于含有0.02% w/v叠氮钠的 50% w/v 硫酸铵溶液。
4°C下稳定超过4年。
Bottle 3:	木霉纤维素酶标准溶液 (5 mL, ~ 3 U/mL; 准确至见瓶标签) 溶于含有0.02% w/v叠氮钠的50% 的丙三醇水溶液。
-20°C下稳定超过4年。

优点：

所有试剂可稳定超过4年
价格非常具有竞争力
完全针对纤维素酶（1,4-葡聚糖内切酶)
常规操作，非常灵敏
检测方法十分简单，适合仪器分析
包含标准

参考文献：

Novel substrates for the measurement of endo-1,4-β-glucanase (endo-cellulase). McCleary, B. V., Mangan, D., Daly, R., Fort, S., Ivory, R. & McCormack, N. (2014). Carbohydrate Research, 385, 9-17.

Quantitative fluorometric assay for the measurement of endo-1,4-β-glucanase. Mangan, D., McCleary, B. V., Liadova, A., Ivory, R. & McCormack, N. (2014). Carbohydrate Research, 395, 47-51.

A novel automatable enzyme-coupled colorimetric assay for endo-1,4-β-glucanase (cellulase). Mangan, D., Cornaggia, C., McKie, V., Kargelis. T. & V. McCleary, B. V. (2016). Analytical and Bioanalytical Chemistry, 408(15), 4159-4168.

说明书下载地址:https://secure.megazyme.com/files/Booklet/K-CellG5_DATA.pdf

内切纤维素酶检测试剂盒

英文名：Cellulase Assay Kit (CELLG3 Method)

货号：K-CELLG3

规格：180 / 360 assays per kit / 720 (auto-analyser)

纤维素酶是一种重要的酶产品，是一种复合酶，主要由外切β-葡聚糖酶、内切β-葡聚糖酶和β-葡萄糖苷酶等组成，还有很高活力的木聚糖酶。

提供内切纤维素酶检测试剂盒，色度法（400 nm）测定酶制品和发酵产品中的纤维素酶（内切-1,4-β-葡聚糖酶）。

内切纤维素酶检测试剂盒(CELLG3方法) K-CELLG3

使用高纯度β-葡萄糖苷酶和苯亚甲基阻断，2 – 氯-4 – 硝基苯基-β-Dcellotrioside（BClPNPβ-G3）。 β-葡萄糖苷酶的能力确保测定的可靠的最大的灵敏度。BClPNPβ-G3的水解为苯亚甲基，通过纤维素酶阻断纤维二糖和2-氯-4 -硝基苯基-β-D-葡萄糖， 2-Cl-4-硝基苯基-β-D-葡萄糖通过β-葡萄糖苷酶立即裂解为D-葡萄糖和游离的2 – 氯-4 – 硝基苯酚（ClPNP）。

反应时间：16min

适用样品：酶制品和发酵产品

优点：

价格低廉（每次检测成本）

特异性高

方法简单

包含标准品

The CELLG3 assay reagent for the measurement of endo-cellulase (endo-1,4-β-glucanase) contains two components;

1) 4,6-O-benzylidene-2-chloro-4-nitrophenyl-β-D-cellotrioside (BCNPG3) and 2) thermostable β-glucosidase. The benzylidene blocking group prevents any hydrolytic action by the β-glucosidase on BCNPG3. Incubation with an endo-cellulase generates a non-blocked colourimetric oligosaccharide that is rapidly hydrolysed by the ancillary β-glucosidase. The rate of formation of 2-chloro-4-nitrophenol is therefore directly related to the hydrolysis of BCNPG3 by the endo-cellulase. The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH 9.0).

Please note that a new assay kit (K-CELLG5) is now available for the measurement of endo-cellulase. The CELLG5 reagent contains a cellopentaose core and exhibits vastly improved sensitivity for some cellulases. In addition, the exchange of the benzylidene blocking group in CELLG3 for 3-keto-butylidene in CELLG5 improves the substrate’s water solubility significantly, allowing for a reduction in the concentration of DMSO required in the assay. As DMSO is known to inhibit certain cellulases, this is another benefit in using CELLG5. Megazyme now recommends the use of K-CELLG5 for all assays for the measurement of endo-cellulase.

Colourimetric method for the determination of
endo-1,4-β-glucanase (cellulase) in enzyme preparations and fermentationproducts

Principle:
(endo-1,4-β-glucanase)
(1) 3-Ketobutylidene-G₅-β-PNP + H₂O → Blocked-G_X + G_(5-X)-β-PNP

(thermostable β-glucosidase)
(2) G_(5-X)-β-PNP + H₂O → D-glucose + PNP

(alkaline solution)
(3) PNP → phenolate ion (yellow colour)
Note: PNP = 4-nitrophenol

Kit size:
K-CELLG5-4V 120 / 240 assays (manual) / 480 (auto-analyser)
or
K-CELLG5-2V 60 / 120 assays (manual) / 240 (auto-analyser)

Method:                      Spectrophotometric at 400 nm
Total assay time:        10 min
Detection limit:                3.5 x 10^-4 U/mL
Application examples:
Fermentation broths, industrial enzyme preparations, biofuels research
Method recognition:     Novel method

Beta葡聚糖[混联]检测试剂盒

β-葡聚糖[混联]检测试剂盒

产品货号：K-BGLU

产品品名：β-葡聚糖[混联]检测试剂盒

英文品名：β-Glucan (Mixed Linkage) Assay Kit

规格型号：100 assays per kit

β-葡聚糖是用独特的工艺开发的一种新的产品，其来源于新鲜的食品啤酒酵母。它是一种多糖，主要化学结构β-1，3 葡聚糖和β-1，6葡聚糖，其中前者具有抗肿瘤性质，而且能够极大地提高人体自然免疫力。

上海金畔生物科技有限公司提供β-葡聚糖[混联]检测试剂盒，比色法（510nm）测定谷物、饲料、食品、饮料和其他原料中的B-葡聚糖。

原理：

总检测时间：100min

检测限：样品重量的0.5-100%

适用样品：燕麦、大麦、麦芽、麦芽汁、啤酒、食品以及其他原料

优点：

成本低

提供的所有试剂稳定性>2年

只有酶试剂盒可用

特异性高

方法简单

包含标准品

方法认证：AOAC (Method 995. 16)、AACC (Method 32-23),EBC (Methods 3.11.1,8.16.l 和 8.11. 1) ICC(标准号l66)，and RACI（标准方法）

分析物意义：大麦和燕麦的主要细胞壁多糖

Megazyme检测试剂盒优点：反应迅速、试剂稳定，只有酶检测试剂盒可用。 AOAC方法 995.16; AACC 方法 32-23

AACC Method 32-23.01, AOAC Method 995.16, EBC Methods 3.11.1, 4.16.1 and 8.11.1, ICC Standard No. 166 and RACI standard method for the measurement of 1,3:1,4-ß-D-glucan in cereal grains, milling fractions, wort and beer. Recommended/Standard procedure of the Association of Official Analytical Chemists (AOAC), American Association of Cereal Chemists (AACC), Royal Australian Chemical Institute (RACI), European Brewing Convention (EBC), and International Association for Cereal Science and Technology (ICC). Content:100 assays per kit

Colourimetric method for the determination of β-Glucan in
cereal grains, feed, foodstuffs, beverages and other materials

Principle:
(lichenase)
(1) β-Glucan + H₂O → β-gluco-oligosaccharides

(β-glucosidase)
(2) β-Gluco-oligosaccharides + H₂O → D-glucose

(glucose oxidase)
(3) D-Glucose + H₂O + O₂ → D-gluconate + H₂O₂

(peroxidase)
(4) 2H₂O₂ + p-hydroxybenzoic acid + 4-aminoantipyrine →
quinoneimine + 4H₂O

Kit size: 100 assays
Method: Spectrophotometric at 510 nm
Total assay time: ~ 100 min
Detection limit: 0.5-100% of sample weight
Application examples:
Oats, barley, malt, wort, beer, food and other materials
Method recognition:
AOAC (Method 995.16), AACC (Method 32-23.01), EBC (Methods
3.10.1, 4.16.1 and 8.13.1), ICC (Standard No. 166), RACI (Standard
Method) and CODEX (Type II Method)

Advantages

Very cost effective
All reagents stable for > 2 years as supplied
Only enzymatic kit available
Very specific
Simple format
Mega-Calc™ software tool is available from our website for hassle-free raw data processing
Standard included

REFERENCES:

McCleary, B. V. and Glennie-Holmes, M. (1985). Enzymic quantification of (1-3)(1-4)-β-D-glucan in barley and malt. J. Inst. Brew. 91, 285-295.
McCleary, B. V. and Nurthen, E. J. (1986). Measurement of (1-3)(1-4)-β-D-glucan in malt, wort and beer. J. Inst. Brew.92, 168-173.
McCleary, B. V. and Codd, R. (1991). Measurement of (1-3) (1-4)-β-D-glucan in barley and oats: a streamlined enzymic procedure. J. Sci. Fd. Agric. 55, 303-312.
McCleary, B. V. and Mugford, D. C. (1992). Interlaboratory evaluation of β-glucan analysis methods. In “The changing role of oats in human and animal nutrition”. Proceedings of the Fourth International Oat Conference, Adelaide, Australia. Oct 19-23.

Beta葡聚糖[酵母和蘑菇]检测试剂盒

Beta葡聚糖[酵母和蘑菇]检测试剂盒 β-Glucan Assay Kit (Yeast & Mushroom)

产品名称：Beta葡聚糖[酵母和蘑菇]检测试剂盒

英文名称：Mushroom and yeast beta-glucan assay Kit

货号： K-YBGL

型号规格：100次

优点：成本低；所有试剂配制后的稳定性〉2年；只有酶试剂盒可用；；方法简单；包含标准品

检测原理：

检测方法：比色法

检测波长：510nm；

总检测时间：100min；

检测限：样品重量的1-100%；

适用样品：酵母制备、蘑菇制备及其他原料；

方法认证：新方法

商品说明：

For the measurement of 1,3:1,6-ß-glucan and α-glucan in yeast preparations. Content: 100 assays per kit

Colourimetric method for the determination of Yeast and
Mushroom β-Glucan in yeast, mushroom, foodstuffs and
other materials

Principle:
(conc. HCl, 30°C, 45 min)
(1) 1,3:1,6-β-Glucan + 1,3-β-glucan + α-glucan + H₂O →
soluble glucan

(1.3 M HCl, 100°C, 2 h)
(2) Soluble glucan + H₂O → D-glucose + laminarisaccharides (trace)

(exo-1,3-β-glucanase + β-glucosidase)
(3) Laminarisaccharides + H₂O → D-glucose

(glucose oxidase)
(4) D-Glucose + H₂O + O₂ → D-gluconate + H₂O₂

(peroxidase)
(5) 2H₂O₂ + p-hydroxybenzoic acid + 4-aminoantipyrine →
quinoneimine + 4H₂O

(amyloglucosidase)
(6) α-Glucan + H₂O → D-glucose

Kit size:                            100 assays
Method:                            Spectrophotometric at 510 nm
Total assay time:              ~ 100 min
Detection limit:                1-100% of sample weight
Application examples:
Yeast preparations, mushroom preparations and other materials
Method recognition:        Novel method

Advantages

Very cost effective
All reagents stable for > 12 months after preparation
Only enzymatic kit available
Simple format
Mega-Calc™ software tool is available from our website for hassle-free raw data processing
Standard included

D-木糖检测试剂盒 D-Xylose assay Kit

商品说明：

The D-Xylose test kit is a novel method for the specific, convenient and rapid measurement and analysis of D-xylose in plant extracts, culture media/supernatants and other materials.

Suitable for manual, auto-analyser and microplate formats.

UV-method for the determination of D-Xylose in fermentation
broths and hydrolysates of plant material and polysaccharides

Principle:
(xylose mutarotase)
(1) α-D-Xylose ↔ β-D-xylose

(β-xylose dehydrogenase)
(2) β-D-Xylose + NAD⁺ → D-xylonic acid + NADH + H⁺

Kit size: 100 assays (manual) / 1000 (microplate)
/ 1300 (auto-analyser)
Method: Spectrophotometric at 340 nm
Reaction time: ~ 6 min
Detection limit: 0.7 mg/L
Application examples:
Analysis of D-xylose in fermentation broths and hydrolysates of plant
material and polysaccharides
Method recognition: Novel method

Advantages

Very cost effective
All reagents stable for > 2 years after preparation
Only enzymatic kit available
Rapid reaction (~ 6 min)
Mega-Calc™ software tool is available from our website for hassle-free raw data processing
Standard included
Suitable for manual, microplate and auto-analyser formats

淀粉总量检测试剂盒

淀粉总量检测试剂盒 Total Starch (AA/AMG) Assay Kit

品牌：Megazyme

产品货号：K-TSTA

品牌：Megazyme

产品品名：淀粉总量检测试剂盒

英文品名：Total Starch (AA/AMG) Assay Kit

规格型号：100 assays per kit

商品说明：

淀粉总量检测试剂盒 Total Starch (AA/AMG) Assay Kit 使用说明：

简介:

酸水解法和酶法被广泛的应用于淀粉含量测定,酸水解法仅仅适用于纯净的淀粉样品,应用范围有限。酶法的前处理步骤上有各种变化，经过凝胶、液化和糊精化作用,糊精水解生成葡萄糖,最终以测量的葡萄糖计算淀粉含量。AACC方法76-11特殊之处在于：在高压蒸汽和碱性环境下淀粉糊化成为凝胶，然后再用淀粉葡糖苷酶将淀粉转化为葡萄糖进行测量。AACC方法76-11会低估某些样品的淀粉含量，包括高多糖玉米淀粉和众多的经过加工的谷物产品。今天应用的众多的测定方法都使用了联合处理方法,如在样品处理过程中或直接在淀粉凝胶化后使用耐热α-淀粉酶。对于难凝胶的样品(如高多糖玉米淀粉)使用了氢氧化钠或二甲基亚砜作为溶剂。为确保膳食纤维中的淀粉能够全部溶解，Englyst和Cummings(1988)总结的处理方法中使用了去分支酶,支链淀粉酶。

为了测量极端样品得到满意结果，兼顾数量和可靠性，Megazyme提供了一个总淀粉分析试剂盒，基于使用耐热性淀粉酶和淀粉葡糖苷酶。这一方法已被AOAC和AACC采用 (Method 76.13)。

最近，耐热淀粉酶可在低pH环境下使用。因此，我们更新了我们的总淀粉测量方法加入了这种酶。这一改进的最大益处在于可以在同一pH（pH5.0）条件下进行耐热性淀粉酶和淀粉葡糖苷酶孵育，简化步骤以及减少麦芽酮糖（抗耐热性淀粉酶和淀粉葡糖苷酶水解）的产生。另一改进是使用己糖激酶/葡萄糖-6-磷酸脱氢酶/ NADP+在D-葡萄糖处理步骤(K-TSHK5)。Megazyme总淀粉分析方法可以测量广泛范围内食品，饲料，植物和谷类产品（自然的或加工的）。对于大多数样品（例如小麦面粉），淀粉可以在100°C抗耐热性淀粉酶处理步骤中完全可溶。包含高水平抗性淀粉（例如高多糖含量玉米淀粉）样品，需要先用冷2 M KOH 或热DMSO进行预溶解。含可溶性淀粉和糊精的样品，无需进行抗耐热性淀粉酶处理。

原理:

抗耐热性淀粉酶水解淀粉为可溶性分支麦芽糊精和去分支麦芽糊精。

样品中的抗性淀粉可用2M KOH进行预溶解，再用醋酸钠缓冲液进行中和再进行淀粉酶水解。或者用DMSO在100°C进行溶解。

淀粉葡萄糖苷酶水解麦芽糊精至D-葡萄糖。

D-葡萄糖氧化为D-葡萄糖酸脂，释放出(H2O2)，再用过氧化氢酶催化进行显色反应，产生醌亚胺染料。

样品包含高水平的D-葡萄糖和麦芽糊精可以在分析前用80%乙醇进行洗涤。单个样品可在70分钟内完成测定。20个样品可在2小时内完成。

特异性，灵敏度，线性范围和精确性

此试剂盒专用于分析a-葡聚糖（包括淀粉，糖原，植物糖原和非抗性麦芽糊精）。

最小可区分的吸收值是0.01吸收值范围。这对应于1.0mg的D-葡萄糖（或0.9mg淀粉）/L在最大样品体积1ml。检测极限：2.0mgD-葡萄糖（或1.8mg淀粉）/L，这对应于0.02吸光度区别在最大体积1ml中。

此试剂盒在5-100ugD-葡萄糖范围内呈线性。一个样品溶液的重复测定中，吸收值会有0.005至0.010的波动。当样品体积为1ml时，这相当于0.05-1ml/L浓度的D-葡萄糖。如果样品在准备过程中被稀释，结果需要乘以稀释倍数。例如在样品准备时，样品称重，如10g/L，变异范围将在0.02至0.05g/100g。

干扰:

如果D-葡萄糖的转化在5分钟内完成，不会产生干扰。可以通过加入D-葡萄糖至反应完成后试管内（例如50ug在0.1ml）以检测干扰。可以看到吸光度显著上升。

样品内的干扰物质可以通过加入内标进行分析。可以对这标准进行定量校正。样品操作中的损失可以通过在起始步骤中加入D-葡萄糖进行校正。

试剂盒成分

Megazyme总淀粉含量测定试剂盒提供有足够运行100次试验的试剂,并提供有全部的试验方法:

瓶1: 耐热α-淀粉酶(10mL, 3,000U/mL在CERALPHA试剂中pH6.5; 40°C 或1600U/mL在CERALPHA试剂中pH5.0; 40°C)。稳定性>4年, 4°C保存。

瓶2: 淀粉葡糖苷酶(10mL, 3300U/mL在可溶性淀粉)（或200U/mL在p-nitrophenylβ-maltoside*，pH4.5，40°C）稳定性>4年, 4°C保存。

完整的分析程序可咨询客服。

瓶3: GOPOD试剂缓冲液。磷酸钾缓冲液（0.26M，pH7.4），对羟基苯甲酸（0.22M），叠氮化钠（0.4%W/V）稳定性>4年, 4°C保存。

注意：

1. 如果GOPOD缓冲液存储在-20°C，会形成盐结晶，用双蒸水稀释至1L前必须完成溶解。

2. 此溶液含叠氮化钠（0.4%W/V），有毒。

瓶4: GOPOD试剂酶。葡萄糖氧化酶(> 12,000 U) 和过氧化物酶(> 650 U)和4-氨基安替比林（80mg）。冻干粉，稳定性>4年, -20°C保存。

瓶5: 葡萄糖标准溶液（5ml，1mg/mL）在0.2% W/V苯甲酸溶液中。稳定性>4年，室温保存。

瓶6: 标准的玉米淀粉。淀粉含量见标签。稳定性>4年, -20°C保存。

准备试剂溶液

溶液1 用试剂1（100mM醋酸钠缓冲液, pH5.0，自备）稀释1.0mL瓶1成分至30mL 。分成小份后冻存。稳定性>3年, -20°C保存。

注意：如果按照AOAC方法996.11（example b），用试剂4（50mM MOPS缓冲液,pH7.0）稀释酶。

溶液2 此酶不用稀释可以直接使用,由于酶溶液具有一定粘稠度,所以必须使用正确的移液器。4°C下保存,稳定性>3年。

溶液3 用蒸馏水稀释瓶3成分（GOPOD试剂缓冲液）到1L, 立即使用。

溶液4 用20ml溶液3溶解瓶4成分再倒回溶液3瓶子。铝箔纸包裹避光。这就是　　葡萄糖测定试剂(GOPOD试剂) 稳定性: 4°C下保存，3个月；-20°C下保存，　　12个月；

分装成小份再进行冻存，只能冻融一次。

新鲜配制的试剂呈浅黄色或浅紫色。4°C下保存2-3个月过程中，逐渐变成深紫色。用水为空白对照时，此溶液的吸光度<0.05。

溶液5&6 同瓶5&6

自备试剂

1.醋酸钠缓冲液(100mM, pH5.0) +氯化钙(5mM)

5.8ml冰醋酸(1.05g/mL)加入900mL蒸馏水,用1M(4g/100mL)NaOH调节pH到5.0,大约需要30mL。4°C下保存2个月.

添加0.74g二水氯化钙完全溶解, 定容到1L, 4°C下保存>6个月。

注意：可通过添加叠氮化钠（0.2g/L）增加此缓冲液的稳定性。室温2年。必须在pH调完后再加入叠氮化钠。酸化的叠氮化钠会释放出有毒气体。

2.醋酸钠缓冲液(1.2M, pH3.8)

69.6ml冰醋酸(1.05g/mL)加入800mL蒸馏水, 用4M NaOH调节pH到3.8, 定容到1L。室温下保存12个月。

3.氢氧化钾溶液（2M）

加入112.2 g KOH 至900 mL 去离子水，搅拌溶解，定容一升。储存于密闭容器。室温下>2年。

4. MOPS缓冲液(50mM,pH7.0)+氯化钙(5mM) +叠氮化钠(0.02%)（仅用于example b分析样品）

11.55g MOPS(钠盐, Sigma cat. M-9381)加入900mL蒸馏水, 用1M(10%V/V)HCl调节pH到7.0,大约需要17mL。添加二水氯化钙(0.74g)和叠氮化钠(0.2g), 完全溶解, 然后调整到1L, 4°C下保存6个月。

5. 醋酸钠缓冲液(200mM,pH4.5)+叠氮化钠(0.02%)（仅用于example b分析样品）

冰醋酸(11.8mL,1.05g/mL)加入900mL蒸馏水, 用1M(4g/100mL)NaOH调节pH到4.5,大约需要60mL。加入叠氮化钠(0.2g),完全溶解,然后调整到1L, 4°C下保存6个月。

注意：可通过添加叠氮化钠（0.2g/L）增加此缓冲液的稳定性。室温2年。必须在pH调完后再加入叠氮化钠。酸化的叠氮化钠会释放出有毒气体。

设备:

　　1.玻璃试管(圆底;16x120mm或18x150mm)

　　2.离心机(3,000rpm)

　　3.微量移液器(100uL).

　　4.连续分配器

　　5.分析天平.

　　6.分光光度计510nm.

　　7.漩涡混合器

　　8.定时钟

　　9.沸水浴锅和试管夹

注意事项:

1.GOPOD试剂的孵育时间没有严格规定,但是至少要20分钟,并在60分钟内测定吸光度.

2.每次试验必须设定试剂空白对照和葡萄糖对照(100μg, 四倍)。

a)试剂空白对照: 0.1mL蒸馏水+3.0mLGOPOD溶液

b)葡萄糖对照包括:0.1mL葡萄糖标准溶液(100μg/0.1mL)+3.0mLGOPOD溶液。因子F（页码13和14）通过D-葡萄糖在标准分析中读得的吸光度进行计算（例如100/1.038=96.386）。

3.每次试验必须设定标准面粉或淀粉样品

4.每一新批此的GOPOD溶液,必须验证其与100μg葡萄糖标准反应产生颜色所需要的最长时间, 通常为15分钟左右。

样品空白:

　样品空白按照标准试验程序（example A）进行,将第4步修正为添加3mL蒸馏水,第5步中也用蒸馏水替代其中的淀粉葡糖苷酶.也可以用样品前处理中所用的酒精作为样品空白( example E)。

样品准备示例

A.不含抗性淀粉，D-葡萄糖和麦芽糊精的谷物和食物（建议的程序，pH5.0孵育）。

1.研磨谷物, 过0.5mm筛（35目）。

2.将研磨后样品(准确称量~100mg)加入到试管中(16x120mm),确保全部样品位于试管底部。

3.加入0.2mL乙醇溶液(80%v/v)湿润样品帮助分散, 用漩涡混合器混合。

4.立即加入3mL耐热α-淀粉酶(100mM醋酸钠稀释), 在沸水浴中孵育6分钟(在孵育2分钟,4分钟，6分钟的时强烈振荡试管).

注意:在这一步中强力震荡是关键，目的是确保浆状样品能完全的混匀（去除块状）。同样,每间隔2分钟振荡也是为了防止由于酒精蒸发导致某些样品被喷出试管。如果使用聚丙烯管，增加孵育时间至12分钟，在 4, 8和12强烈震荡。

5.将试管放置于50°C的水浴锅中, 加入0.1mL瓶2成分（淀粉葡糖苷酶,330U在淀粉上）。充分混合,在50°C下孵育30分钟。

6.将全部的溶液转移到100mL的容量瓶中,用洗瓶冲洗试管,并也倒入容量瓶中,然后用蒸馏水调整溶液体积, 充分混匀。取部分溶液离心(3,000rpm,10分钟)。取清澈的未稀释的上清用于分析。

注意：对于含有1-10%淀粉的样品,在第6步中用蒸馏水调整溶液至10mL,充分混匀,离心(3,000rpm,10分钟),该溶液可以直接进行第7步.对于含10-100%淀粉的样品,用蒸馏水稀释1.0mL样品液到10mL再进行第7步。

7.转移两份稀释的样品溶液(0.1mL)到园底试管中(16x100mm)。

8.加入3.0mLGOPOD溶液到每一个试管中(包括葡萄糖对照和试剂空白对照), 然后在50°C下孵育20分钟

9.葡萄糖对照包括0.1mL葡萄糖标准溶液(1mg/mL)+3.0mLGOPOD溶液。　　试剂空白对照: 0.1mL蒸馏水+3.0mLGOPOD溶液

10.相对于试剂空白在510nm下测定每一个样品和葡萄糖质控的吸光度。

B.不含抗性淀粉，D-葡萄糖和麦芽糊精的谷物和食物（AOAC 996.11）。

1.研磨谷物, 过0.5mm筛（35目）。

2.将研磨后样品(准确称量~100mg)加入到试管中(16x120mm),确保全部样品位于试管底部。

3.加入0.2mL乙醇溶液(80%v/v),用漩涡混合器混合。

4.立即加入3mL耐热α-淀粉酶(50mM MOPS稀释), 在沸水浴中孵育6分钟(在孵育2分钟,4分钟，6分钟的时强烈振荡试管).

5.将试管放置于50°C的水浴锅中, 加入醋酸钠缓冲液（4ml，200mM，pH4.5），加入0.1mL瓶2成分（淀粉葡糖苷酶, 20U）。充分混合,在50°C下孵育30分钟。

6. 按照example A 步骤6继续实验。

C.含抗性淀粉，不含D-葡萄糖和麦芽糊精的谷物和食物（建议KOH溶解）

1.研磨谷物, 过0.5mm筛（35目）。

2.将研磨后样品(准确称量~100mg)加入到试管中(16x120mm),确保全部样品位于试管底部。

3.加入0.2mL乙醇溶液(80%v/v),用漩涡混合器混合。

4.放入搅拌架子，加入2ml 2M KOH至每个试管中，冰水混合浴中搅拌20分钟左右，重悬粉末和溶解抗性淀粉。

注意：

1.不要使用涡旋仪混匀，会使淀粉乳化。

2.确保加入KOH时，试管处于剧烈震荡状态。这是为了避免淀粉形成团块难以溶解。

5.当试管在磁力搅拌器上搅拌时，加入8ml 1.2M醋酸钠缓冲液（pH3.8）至每个试管。立即加入0.1ml耐热α-淀粉酶（瓶1）和0.1mL淀粉葡糖苷酶（瓶2），充分混合,在50°C下孵育

6. 孵育30分钟，间断混匀。

7. 样品淀粉含量>10%：将全部的溶液转移到100mL的容量瓶中,用洗瓶冲洗试管,并也倒入容量瓶中,然后用蒸馏水调整溶液体积至100ml, 充分混匀。取部分溶液离心(1800g,10分钟)。

8. 样品淀粉含量<10%：直接离心(1800g,10分钟)。总体积约10.4ml（此体积可能有一定波动，特别是湿样品用于分析时，估计体积用于计算）。

9. 按照example A 步骤7继续实验。

D.含抗性淀粉，不含D-葡萄糖和麦芽糊精的谷物和食物（AOAC 996.11 DMSO溶解）

1.研磨谷物, 过0.5mm筛（35目）。

2.将研磨后样品(准确称量~100mg)加入到试管中(16x120mm),确保全部样品位于试管底部。

3.加入0.2mL乙醇溶液(80%v/v),用漩涡混合器混合。

4.立即加入2ml DMSO，涡旋混匀。沸水浴5分钟。

5.按照example A 步骤4继续实验。

E.含D-葡萄糖和麦芽糊精的谷物和食物

1.研磨谷物, 过0.5mm筛（35目）。

2.将研磨后样品(准确称量~100mg)加入到试管中(16x120mm),确保全部样品位于试管底部。

3.加入5mL乙醇溶液(80%v/v)，80-85°C孵育5分钟。涡旋混匀，再加入5ml乙醇溶液(80%v/v)。

4.离心1800g（3000rpm）10分钟。去除上清。

5.10mL乙醇溶液(80%v/v)重悬沉淀。离心去除上清。

6. 按照example A 步骤4继续实验。

若样品含抗性淀粉，按照example C步骤4继续实验。

计算:

ΔA=相对于试剂空白所读取的吸光度

FV=总体积（例如100ml或10ml）

0.1 =样品分析体积

1/1000=转化从ug至mg

100/w=淀粉作为干粉重量的比例。

W=干粉重量

162/180=D葡萄糖转化为脱氢葡萄糖

淀粉%(占干物质比重):＝淀粉%X100/100-水分含量(%)

英文说明：

The Total Starch (AA/AMG) test kit is used for the measurement and analysis of total starch in cereal flours and food products. This kit now contains an improved α-amylase that allows the amylase incubations to be performed at pH 5.0 (as well as pH 7.0).

Colourimetric method for the determination of Total Starch in
cereal products, feeds, foodstuffs and other materials

Principle:
(α-amylase, 100°C ± DMSO)
(1) Starch granules + H₂O → maltodextrins

(amyloglucosidase)
(2) Maltodextrins + H₂O → D-glucose

(glucose oxidase)
(3) D-Glucose + H₂O + O₂ → D-gluconate + H₂O₂

(peroxidase)
(4) 2H₂O₂ + p-hydroxybenzoic acid + 4-aminoantipyrine →
quinoneimine + 4H₂O

Kit size: 100 assays
Method: Spectrophotometric at 510 nm
Total assay time: ~ 90 min
Detection limit: 1-100% of sample weight
Application examples:
Cereal flours, food products and other materials
Method recognition:
AOAC (Method 996.11), AACC (Method 76-13.01), ICC (Standard Method
No. 168), and RACI (Standard Method)

Advantages

Very competitive price (cost per test)
All reagents stable for > 12 months after preparation
Simple format
Mega-Calc™ software tool is available from our website for hassle-free raw data processing
Standard included

蔗糖/D-果糖/D-葡萄糖检测试剂盒

蔗糖/D-果糖/D-葡萄糖检测试剂盒 Sucrose/Fructose/D-Glucose Assay Kit

产品名称：蔗糖/D-果糖/D-葡萄糖检测试剂盒

英文名称：Sucrose,D-fructose and D-glucose sucrose assay kit

货号：K-SUFRG

型号规格：150 (50 of each)

优点：价格低廉（每次检测成本）；

所有试剂配制后的稳定性〉12个月；

反应快；葡萄糖氧化酶/过氧化酶/己糖激酶/6-磷酸葡萄糖脱氢酶；

包含标准品

检测原理：

检测方法：紫外分光光度法

检测波长：340nm；

反应时间：30min；

检测限：1.38mg/L；

适用样品：啤酒、果汁、软饮料、牛奶、果酱、蜂蜜、面包、焙烤产品、乳制品、糖果、甜点、糖食、冰淇淋、水果和蔬菜（如：马铃薯）、肉制品（如香肠）、调味品、饲料、烟、化妆品、纸及其他原料（如生物培养、样品等）；

方法认证：EN。NEN。NF。DIN，GOST，IFU ,AIJN,MEI，IOCCC以及德国、奥地利认证

商品说明：

The Sucrose/Fructose/D-Glucose test kit is suitable for the measurement and analysis of sucrose, D-glucose and D-fructose in plant and food products.

Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4^oC.

UV-method for the determination of Sucrose, D-Fructose and
D-Glucose in foodstuffs, beverages and other materials

Principle:
(β-fructosidase)
(1) Sucrose + H₂O → D-glucose + D-fructose

(hexokinase)
(2) D-Glucose + ATP → G-6-P + ADP

(hexokinase)
(3) D-Fructose + ATP → F-6-P + ADP

(glucose-6-phosphate dehydrogenase)
(4) G-6-P + NADP⁺ → gluconate-6-phosphate + NADPH + H⁺

(phosphoglucose isomerase)
(5) F-6-P → G-6-P

Kit size: 100 assays of each
Method: Spectrophotometric at 340 nm
Reaction time: ~ 30 min
Detection limit: 1.38 mg/L
Application examples:
Beer, fruit juices, soft drinks, milk, jam, honey, dietetic foods, bread,
bakery products, dairy products, candies, desserts, confectionery, sweets,
ice-cream, fruit and vegetables (e.g. potato), meat products (e.g. sausage),
condiments (e.g. ketchup and mustard), feed, tobacco, cosmetics,
pharmaceuticals, paper and other materials
Method recognition:
Methods based on this principle have been accepted by NF, EN, NEN,
DIN, GOST, IFU, AIJN, MEBAK and IOCCC

Advantages

Very competitive price (cost per test)
All reagents stable for > 2 years after preparation
Rapid reaction
Mega-Calc™ software tool is available from our website for hassle-free raw data processing
Stabilised D-glucose / D-fructose standard solution included
Extended cofactors stability