Megazyme酶试剂 | 生化试剂|生物酶|标准品

Megazyme E-MALBS 低聚α- （ 1,4-1,6 ）葡糖苷酶（芽孢杆菌）

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Megazyme E-MALBS 低聚α- （ 1,4-1,6 ）葡糖苷酶（芽孢杆菌）

中文名称：低聚α- （ 1,4-1,6 ）葡糖苷酶（芽孢杆菌）
英文名：Oligo-α-(1,4-1,6)-glucosidase (Bacillus sp.)

货号：E-MALBS

规格：3,000 Units at 40oC

High purity oligo-α-(1,4-1,6)-glucosidase (Bacillus sp.) for use in research, biochemical enzyme assays and in vitrodiagnostic analysis.

EC 3.2.1.10
CAZy Family: GH13
CAS: 9032-15-9

oligo-1,6-glucosidase; oligosaccharide 6-alpha-glucohydrolase

Recombinant. From Bacillus sp.
In 3.2 M ammonium sulphate.

Specific activity: 87 U/mg protein (on p-nitrophenyl-α-D-glucopyranoside) at pH 7.0 and 40oC.

Stability: > 2 years 4oC.

爱尔兰Megazyme是一家全球的制造和供应高品质和创新的检测谷物，食品，饲料，发酵，乳制品和葡萄酒行业的技术。Megazyme成立于1988年，该公司目前提供了超过70种试剂盒和超过300种其他试剂和底物。

葡萄糖苷酶是糖苷水解酶大家族中的一大类酶，主要功能为水解葡萄糖苷键，释放出葡萄糖作为产物，是生物体糖代谢途径中不可或缺的一类酶。Megazyme品牌岩藻糖苷酶主要用于糖生物学研究。根据不同葡萄糖苷酶对寡糖底物的水解方式，可将期分为外切（exo-）葡萄糖苷酶与内切（endo-）葡萄糖苷酶。外切葡萄糖苷酶是指从寡糖底物的一端（还原端或非还原端）进行水解的葡萄糖苷酶，而内切葡萄糖苷酶则是指从寡糖底物的中间部分开始水解的葡萄糖苷酶。葡萄糖苷酶因为其特性，主要应用于两个方面，1）纤维素的水解与利用：主要涉及各种β-葡萄糖苷酶与纤维素水解相关酶类，目的即将难溶的纤维素变为可溶的、易于利用的小分子寡糖。2）功能性低聚糖的合成：主要涉及葡萄糖苷酶的转糖苷活力，目的即通过具有转苷活力的葡萄糖苷酶合成功能性低聚葡聚糖、低聚麦芽寡糖、低聚纤维寡糖等可以作为益生元的功能性糖类。

Megazyme品牌葡萄糖苷酶系列产品，信息如下：

产品编号	中文名称	英文品名	规格
E-E-AGLUTM	α-葡萄糖苷酶（耐热）（海栖热袍菌）	α-Glucosidase (thermostable) (Thermotoga maritima)	500 Units at 80oC
E-E-MALTS	α-葡萄糖苷酶 (麦芽糖酶)	Alpha-Glucosidase (Maltase)	2,000 Units
E-E-TRNGL	α-葡[萄]糖苷酶 [转葡糖苷酶][黑曲霉]	α-Glucosidase (transglucosidase) (Aspergillus niger)	2,000 Units
E-TSAGL	α-葡萄糖苷酶[嗜热脂肪芽孢杆菌]	α-Glucosidase (Bacillus stearothermophilus)	1,500 Units
E-E-TSAGS	重组α-葡萄糖苷酶[嗜热脂肪芽孢杆菌]	α-Glucosidase (Bacillus stearothermophilus) (Recombinant)	3,000 Units at 40oC; ~ 6,000 Units at 60oC
E-E-MALBS	寡聚α-（1,4-1,6）葡萄糖苷酶（枯草芽孢杆菌）	Oligo-α-(1,4-1,6)-glucosidase (Bacillus sp.)	3,000 Units at 40oC
E-E-OAGUM	低聚α-1,6-葡萄糖苷酶（微生物）	Oligo-α-1,6-Glucosidase (microbial)	3,000 Units at 40oC

Megazyme K-PULLG6 普鲁兰酶/极限醍醐精检测试剂盒

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Megazyme K-PULLG6 普鲁兰酶/极限醍醐精检测试剂盒

中文名称：普鲁兰酶/极限醍醐精检测试剂盒

英文名：Pullulanase/Limit-Dextrinase Assay Kit (PULLG6 Method)

货号：K-PULLG6

规格：100 assays (manual)

PULLG6 assay for the measurement of pullulanase employs a water soluble defined substrate, namely 4,6-O-benzylidene-4-nitrophenyl-6³-α-D-maltotriosyl-maltotriose (BPNPG3G3), coupled with the ancillary enzymes α-glucosidase and β-glucosidase. Upon hydrolysis of the substrate at the 1,6-α-linkage by pullulanase or limit-dextrinase, the released 4-nitrophenyl-β-maltotrioside is immediately hydrolysed to glucose and 4-nitrophenol by the concerted action of the α-glucosidase and β-glucosidase enzymes in the reagent mixture. The reaction is terminated and phenolate ions are developed by addition of dilute alkali. The absorbance is read at 400 nm and the value obtained correlates directly with pullulanase activity.

PullG 6法测定普鲁兰酶采用水溶性底物，即4，6-O-苄基-4-硝基苯-6³-α-D-麦芽糖-丙三糖(BPNPG3G3)，与α-葡萄糖苷酶和β-葡萄糖苷酶结合.当底物在1，6-α-链上被普鲁兰酶或极限糊精酶水解后，释放出的4-硝基苯基-β-麦芽三糖苷在试剂混合物中α-葡萄糖苷酶和β-葡萄糖苷酶的协同作用下，立即水解成葡萄糖和4-硝基酚。反应结束后，加入稀碱，形成酚酸根离子。在400 nm处读取吸光度，得到的吸光度值与普鲁兰酶活性直接相关。

Colourimetric method for the determination of pullanase
or limit-dextrinase

Principle:
(pullulanase/limit-dextrinase)
(1) Benzylidene-G₃-(α-1,6)-G₃-β-PNP + H₂O → Benzylidene-G₃
+ G₃-β-PNP

(thermostable α-glucosidase and β-glucosidase)
(2) G₃-β-PNP + H₂O → D-glucose + PNP

(alkaline solution)
(3) PNP → phenolate ion (yellow colour)

Note: PNP = 4-nitrophenol

Kit size: 100 assays
Method: Spectrophotometric at 400 nm
Total assay time: 10 min for pullanase preparations
30 min for malt extracts containing
limit-dextrinase
Detection limit: 0.18 U/mL for pullulanase preparations
(50-fold dilution)
0.01 U/g for limit dextrinase in milled malt
Application examples: Assay of microbial pullulanase preparations
Measurement of limit-dextrinase in
malt extracts
Method recognition: Novel method

Advantages

High sensitivity
Suitable for manual and auto-analyser formats
No transglycosylation interference
Very cost effective
All reagents stable for > 1 year after preparation
Very specific
Simple format
Standard included

甘油检测试剂盒

甘油检测试剂盒 Glycerol Assay Kit

产品货号：K-GCROL

产品品名：甘油检测试剂盒

英文品名：Glycerol Assay Kit

规格型号： 70 assays per kit

甘油是食品加工业中通常使用的甜味剂和保湿剂，大多出现在运动食品和代乳品中。

The Glycerol test kit is a simple, reliable, rapid and accurate method for the measurement and analysis of Glycerol in beverages, foodstuffs and other materials.
Suitable for manual and microplate formats.

UV-method for the determination of Glycerol in foodstuffs,
beverages and other materials

原理： (glycerokinase)
(1) Glycerol + ATP → L-glycerol-3-phosphate + ADP

(pyruvate kinase)
(2) ADP + PEP → ATP + pyruvate

(L-lactate dehydrogenase)
(3) Pyruvate + NADH + H⁺ → L-lactic acid + NAD⁺

反应时间： 5min

检测限： 0.37mg/L

适用样品： 葡萄酒（葡萄汁）、啤酒、烈酒、醋、杏仁蛋白软糖、果汁、软饮料、牙膏、蜂蜜、烟、纸（硬纸板）、化妆品

优点：

l 新型的片剂形式增加了稳定性

l 价格低廉（每次检测成本）

l 所有试剂配制后的稳定性 >2 年

l 反应快

l 该方法已通过 OIV ， MEBAK 以及德国和瑞士的认证

REFERENCES:

Spinella, C. I. (1966). Modified enzymatic procedure for the routine determination of glycerol and triglycerides in plasma. J. Lipid Res ., 7,167-169 .
Klopper, W. J., Angelino, S. A. G. F., Tuning, B. & Vermeire, H. A. (1986). Organic acids and glycerol in beer. J. Inst. Brew ., 92, 225- 228.
Michal, G. (1976). Enzymatische analyse in der pharmazie. Acta Pharmaceutica Technologica , Suppl. 1, S, 151-162.
Pfandl, A. & Menschig, D. (1984). Ein beitrag zur enzymatischen glycerin- und ethanol-bestimmung. Pharm. Ind ., 46, 403-407.
Wieland, O. H. (1988). Glycerol. In Methods of Enzymatic Analysis (Bergmeyer, H. U., ed.), 3rd ed., Vol. VI, pp. 504-510, VCH Publishers (UK) Ltd., Cambridge, UK.

Megazyme O-PNPX2 p-Nitrophenyl-ß-xylobiose 50mg

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Megazyme试剂盒代理、Megazyme代理

英文名称：4-Nitrophenyl-β-xylobioside

CAS: 6819-07-4
Molecular Formula: C16H21NO11
Molecular Weight: 403.3
Purity: > 97%High purity 4-Nitrophenyl-β-xylobioside for use in research, biochemical enzyme assays and in vitro diagnostic analysis. This is a colourimetric substrate for measurement of β-xylanase and β-xylosidase activity.

柠檬酸检测试剂盒

柠檬酸检测试剂盒, Citric Acid Assay Kit

品牌：Megazyme

货号：K-CITR

中文品名：柠檬酸检测试剂盒

品名：Citric Acid Assay Kit

规格：72次(手工)/720次(微孔板)/840次(自动分析仪)

应用：快速、可靠地检测食品、饮料和其它物料中柠檬酸（柠檬酸盐）含量
原理：

食品、饮料及其他材料中柠檬酸含量的紫外检测方法

(citrate lyase)

(1) Citrate → oxaloacetate + acetate

(L-malate dehydrogenase)

(2) Oxaloacetate + NADH + H+ → L-malate + NAD+

(D-lactate dehydrogenase)

(3) Pyruvate + NADH + H+ → D-lactate + NAD+

检测方法：分光光度法 @340 nm

反应时间：5min

检测限：0.921mg/L

(注意：如果样品中草酰乙酸脱羧酶，将一些草酰乙酸产物转变为丙酮酸：因此，为了确保柠檬酸的定量准确，采用D-乳酸脱氢酶( D-LDH)，将丙酮酸产物转变为D-乳酸和NAD+）

方法识别：

通过MEBAK，OIV，EU，IS02963，AOAC和 IFLU22的认可

应用案例：

葡萄汁、葡萄酒、啤酒、果汁、软饮料、茶、乳制品（如奶酪）、肉、加工过的肉、蔬菜和水果产品、焙烤食品、纸、医药品、化妆品以及其他原料（如生物培养基、样品等）。

试剂盒组成：

Bottle 1: 缓冲溶液 (40 mL, pH 7.5) 添加叠氮钠(0.02%)作为防腐剂
稳定超过 2年 at 4°C
Bottle 2: NADH，添加 PVP.
稳定超过 5年 at -20°C
Bottle 3: L-苹果酸脱氢酶，添加 D-乳酸脱氢酶, 1.5 mL.
稳定超过 2年 at 4°C.
Bottle 4: (x 3) 柠檬酸裂解酶，冻干
稳定超过 2年 at -20°C
Bottle 5: 柠檬酸标准溶液 (5 mL, 0.20 mg/mL)溶于0.02% (w/v)叠氮钠溶液
稳定超过 2年 at 4°C

优点：

重组柠檬酸裂解酶4℃稳定性为4周/ -20℃，6个月
试剂盒组分缓冲液／辅助因子／酶片剂，可有效利用
加入PVP防止丹宁酸的抑制
适用于手工和自动分析仪检测
价格具有竞争力
包含标准溶液

参考文献：

Mollering, H. (1989). Citrate. In Methods of Enzymatic Analysis (Bergmeyer, H. U., ed.), 3rd ed., Vol. VII, pp. 2-12, VCH Publishers (UK) Ltd., Cambridge, UK.

Megazyme L-CONA 刀豆球蛋白A Concanavalin A (Con A)

Megazyme品牌介绍：

爱尔兰Megazyme是生产分析试剂、酶和食品酶法试剂盒的公司，是开发饮料、谷类食品、乳制品、食品、饲料、发酵、生物燃料和葡萄酒行业提供试剂盒和试剂的公司。从公司成立之初的检测试剂盒试剂盒生产，到现在新的领域，碳水化合物的和谷物酶相关行业发展已成为检测领域领导者之一。

Megazyme L-CONA 刀豆球蛋白A Concanavalin A (Con A)

中文名称：刀豆球蛋白A

英文名：Concanavalin A (Con A)

货号：ML-CONA-1000MG

规格：1 g

High purity Concanavalin A (Con A) lectin has a highly specific carbohydrate binding affinity, for research and in vitro diagnostic analysis.

Affinity purified, lyophilised powder. Con A is not blood group specific, has an affinity for terminal α-D-mannose and α-D-glucose residues and requires the presence of Ca²⁺ and Mn²⁺ for activity.

高纯度刀豆蛋白A(ConA)凝集素具有高度特异性的碳水化合物结合亲和力，用于研究和离体分析

亲和纯化冻干粉末。Con A不具有血型特异性，对末端α-D-甘露糖和α-D-葡萄糖残基具有亲和力，需要钙的存在。²⁺锰²⁺为了活动。

DESCRIPTION
(1) FORM
Affinity purified, off-white lyophilised powder.
(2) BIOCHEMICAL / PHYSIOLOGICAL PROPERTIES
Con A is not blood group specific, has an affinity for terminal α-D-mannose and α-D-glucose residues and requires the presence of Ca2+ and Mn2+ for activity. Con A exists in dimeric (pH < 5.6), tetrameric (pH between 5.6 and 7.0) and aggregate (pH > 7.0) forms. An active dimer above pH 5.6 can be generated by succinylation. Con A exhibits mitogenic activity which is dependent upon its degree of aggregation.

(3) PROPERTIES
Activity: 20 μg/mL
Electrophoretic purity: Electrophoresis was preformed using a 14% acrylamide gel.
UV absorbance: In 100 mM NaCl; λmax = 275.8; E1% = 13.7.
Solubility: Slightly hazy, colourless solution at 10 mg/mL in water.
Activity note: Activity is determined using a twofold serial dilution of 1 mg/mL solution of
Con A in PBS* (8.0 g NaCl, 0.3 g KCl, 0.2 g KH2PO4/L; pH 7.2) containing 1
mM Ca2+ and 1 mM Mn2+. The activity is expressed as the lowest concentra-
tion to give agglutination of a 2% suspension of human red blood cells (type O)
in PBS* after 1 hr incubation at room temperature.
(4) STORAGE / STABILITY / SAFETY
Storage temperature: -20ºC
Shelf life: > 3 years at -20ºC
Safety statement: 22-24 / 25

Reference 参考文献：
Entlicher, G.J., Kostir, V. and Kocourek, J. (1971) Studies on phytohemmagglutinins.
8. Isoelectric point and multiplicity of purified concanavalin A. Biochim Biophys Acta 236(3): 795-7

Megazyme D-葡萄糖[GOPOD法]检测试剂盒

Megazyme试剂盒代理、Megazyme代理
欢迎访问Megazyme官网或者咨询我们获取更多产品信息。

中文名称：D-葡萄糖[GOPOD法]检测试剂盒

英文名：D-Glucose (GOPOD Format) Assay Kit

货号：K-GLUC

规格：660 assays per kit

品牌： Megazyme

分析物意义：常见食品组分，在某些情况下非常重要，如糖尿病产品

Megazyme检测试剂盒优点：选择简单可用的方法，葡萄糖氧化酶/过氧化酶 /己糖激酶/6-磷酸葡萄糖脱氢酶。试剂稳定

The D-Glucose test kit contains high purity reagents for the measurement and analysis of D-glucose in cereal extracts and for use in combination with other Megazyme kits.

Colourimetric method for the determination of D-Glucose in
foodstuffs, beverages and other materials

Principle:
(glucose oxidase)
(1) D-Glucose + H₂O + O₂ → D-gluconate + H₂O₂

(peroxidase)
(2) 2H₂O₂ + p-hydroxybenzoic acid + 4-aminoantipyrine →
quinoneimine + 4H₂O

Kit size: 660 assays
Method: Spectrophotometric at 510 nm
Reaction time: ~ 20 min
Detection limit: 100 mg/L
Application examples:
Wine, beer, fruit juices, soft drinks, milk, jam, dietetic foods, bakery
products, candies, fruit and vegetables, tobacco, cosmetics, pharmaceuticals,
feed, paper and other materials (e.g. biological cultures, samples, etc.)
Method recognition:
Widely used and accepted in clinical chemistry and food analysis

Advantages

All reagents stable for > 12 months after preparation
Very competitive price (cost per test)
Simple format
Standard included

FAQ解答

Q1. There is an issue with the performance of the kit; the results are not as expected.

If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:

Ensure that you have tested the standard sample that is supplied with the Megazyme test kit.
Send the results of the kit standard, blank samples and the results obtained for your sample, in the relevant MegaCalc spreadsheet (if available) to Megazyme (cs@megazyme.com). Where available the relevant MegaCalc spreadsheet can be downloaded from where the product appears on the Megazyme website.
State the kit lot number being used (this is found on the outside of the kit box).
State which assay format was used (refer to the relevant page in the kit booklet if necessary).
State exact details of any modifications to the standard procedure that is provided by Megazyme.
State the sample type and describe the sample preparation steps if applicable.

Q2. I have some questions regarding the Glucose Oxidase and peroxidase in the Glucose Assay Kit.

At what optimal pH do the enzymes work? Does the sample that is added to the enzyme mix show incorrect results if it is outside a certain pH range?

The test is best run at pH 7.4. The reagent is buffered at this pH. Using different pH values will affect results, i.e. it may take longer to reach this end point; but the same end-point value should be obtained if not too far away from this pH value.

Q3. What is the linear range of the Glucose Assay Kit?

The test is set up to measure between 10 and 100 micrograms per assay (i.e. 0.1 mL of 0.1 to 1.0 mg/mL). You may prefer to use 3.0 mL of GOPOD reagent mixture plus 1.0 mL of sample; in this case the concentration range in the material being analysed (diluted) should be ~10 to 100 micrograms per mL. The test is linear up to an absorbance of 1.4 (final assay volume of 3.2 mL). If the final volume is 4.0 mL, then linearity will be up to about 1.0 absorbance units.

Q4. I have some questions on your Glucose Assay Kit. Is the colour stable at room temperature? Does the reaction continue if not read within 20 minutes?

The reaction is complete after approx. 15 min, and the colour is stable at temperatures of 20-50˚C for at least an extra hour.

Q5. Please let us know the lower limit of detection of measuring glucose using your Glucose Assay Kit (mg/mL).

1 microgram gives an absorbance of 0.01 if 3 mL of GOPOD are used. If 1 mL of GOPOD is used, 1 microgram gives an absorbance of 0.03 OD.

Q6. Should the pH of the sample be adjusted even for samples in acidic media?

The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.

Q7. Sometimes a negative absorbance change is obtained for the blank samples, is this normal? Should the real value (negative absorbance change) or “0” be used in the calculation of results?

Sometimes the addition of the last assay component can cause a small negative absorbance change in the blank samples due to a dilution effect and in such cases it is recommended that the real absorbance values be used in the calculation of results.

Q8. We stored the Glucose Assay Kit at 0-5°C for some weeks, because that is what is written on the package. Now we have noticed on some of the vials it says storage at –20°C. Can you tell me how long the products can be stored at 0-5°C without damage?

The kit enzymes can be stored at 0-5°C for up to 12 months, so they will be perfectly fine.

Q9. The temperature for the Glucose Assay Procedure (40 or 50˚C)-is this for incubation of the mix or reading the results at the spectrophotometer? Could I read the results at room temperature?

The temperature is just for the incubations. It is an end-point assay, so the spectrophotometer does not need to be temperature controlled.

Q10. Can oligosaccharides or polysaccharides be measured using the kit assay?

The kit assay will only measure the non-covalently linked monosaccharide.

Oligosaccharides or polysaccharides can be measured after hydrolysis to the monosaccharide. Generally acid hydrolysis can be achieved by boiling the oligo/polysaccharide in 1.3 M HCl for 1 h. It is recommended that scientific literature is consulted for information on hydrolysis conditions for the particular oligo/polysaccharide that is being measured.