Megazyme试剂盒 | 生化试剂|生物酶|标准品

Beta葡聚糖酶[麦芽和微生物]检测试剂盒

Beta葡聚糖酶[麦芽和微生物]检测试剂盒 β-Glucanase Assay Kit (Malt and Microbial)

1) 英文：Beta-Glucanase (Malt & Microbial) Assay Kit;

2) 中文：Beta葡聚糖酶[麦芽和微生物]检测试剂盒;

3) 规格: 100; 4) 货号:K-MBGL

商品说明：

β-Glucanase (Malt and Microbial) Assay Kit is suitable for the measurement and analysis of malt and bacterial β-glucanase and endo-1,4-β-glucanase.

Colourimetric method for the determination of β-Glucanase
in malt, foodstuffs and fermentation products

Principle:
(β-glucanase)
(1) Azo-Barley β-glucan (polymer) → Azo-barley β-glucan
(fragments)

(2) Add alcohol; centrifuge to remove polymeric Azo-barley
β-glucan

(3) Measure the absorbance of the supernatant solution

Kit size: 100 assays
Method: Spectrophotometric at 590 nm
Reaction time: ~ 30 min
Detection limit: 100 U/kg of malt
Application examples:
Malt extracts, wort, beer and other materials
Method recognition:
RACI (Standard Method)

Advantages

Very cost effective
All reagents stable for > 2 years during use
Only kit available
Very specific
Simple format
Standard included

D-甘露糖/D-果糖/D-葡萄糖检测试剂盒

D-甘露糖/D-果糖/D-葡萄糖检测试剂盒 D-mannose/D-fructose/D-glucose assay kit

产品名称：D-甘露糖/D-果糖/D-葡萄糖检测试剂盒

英文名称：D-mannose/D-fructose/D-glucose assay kit

货号： K-MANGL

型号规格：55次

优点：成本低（每次检测成本）；所有试剂配制后的稳定性〉2年；只有酶试剂盒可用；方法简单；反应快；包含标准品

检测原理：

检测方法：紫外分光光度法

检测波长：340nm；

反应时间：25min；

检测限：0.66mg/L；

适用样品：食品、酵母细胞制剂、酶水解产物和其他原料（如：生物培养、样品等）；

方法认证：新方法

商品说明：

The D-Mannose/D-Fructose/D-Glucose test kit is suitable for the specific measurement and analysis of D-mannose, D-fructose and D-glucose in plant products and in acid hydrolysates of polysaccharides.

UV-method for the determination of D-Mannose, D-Fructose
and D-Glucosein foodstuffs, yeast cell preparations and
other materials

Principle:
(hexokinase)
(1) D-Mannose / D-fructose / D-glucose + ATP →
M-6-P / F-6-P / G-6-P + ADP

(glucose-6-phosphate dehydrogenase)
(2) G-6-P + NADP⁺ → gluconate-6-phosphate + NADPH + H⁺

(phosphomannose isomerase) (phosphoglucose isomerase)
(3) M-6-P ↔ F-6-P ↔ G-6-P

Kit size: 55 assays
Method: Spectrophotometric at 340 nm
Reaction time: ~ 30 min
Detection limit: 0.7 mg/L
Application examples:
Foodstuffs, yeast cell preparations, enzymatic hydrolysates and other
materials (e.g. biological cultures, samples, etc.)
Method recognition: Novel method

Advantages

Very competitive price (cost per test)
All reagents stable for > 2 years after preparation
Only enzymatic kit available
Simple format
Rapid reaction
Mega-Calc™ software tool is available from our website for hassle-free raw data processing
Standard included

D-乳酸检测试剂盒，D-Lactic Acid Assay Kit

名称:D-乳酸检测试剂盒
英文名:D-Lactic Acid Assay Kit
规格:50次
产地:爱尔兰
外观：五玻璃瓶。
比重：不适用
在水中的溶解度：易溶
pH值： 7.0
气味：无
粉末和液体形式
稳定性: 稳定 5年或以上(冷冻)

成分:
名称比例
甘氨酰甘氨酸缓冲液 1瓶（玻璃）
烟酰胺腺嘌呤二核苷酸 1瓶（玻璃）
D-谷氨酸丙酮酸转氨酶 1瓶（玻璃）
D-乳酸脱氢酶酶 1瓶（玻璃）
D-乳酸的标准品 1瓶（玻璃）

许多食品和饮料中都发现有D-乳酸. 由乳酸菌产生, D-乳酸存在于牛奶的发酵产品中比如酸奶，腌菜，腌肉和腌鱼中。牛奶、肉、和果汁的质量可以通过测定D-乳酸的含量来确定。在葡萄酒生产中，D-乳酸的产生标志着酒被乳酸菌***。在化学工业中，D-乳酸和L-乳酸都是生产过程中聚合物的原料，比如聚交酯和一些生物学上可降解的聚合物。同时也应用在化妆品和医药品中。

原理:

D-乳酸的定量分析需要两步酶化反应。第一个反应由D-乳酸脱氢酶(D-LDH)催化,D-乳酸在烟酰腺嘌呤二核苷酸(NAD+)的催化下，被氧化成丙***酸

然而, 因为反应(1)的移动和D-乳酸和NAD+有着密切的关系，下一步反应需要消耗掉产生的丙***酸在大量D-谷氨酸的存在下，通过在D-谷氨酸-丙***酸转氨酶（D-GPT）的作用下把丙***酸转化为D-丙氨酸和2-***戊二酸。(2).

以上两个反应中产生的 NADH 的量可以用来计量D-乳酸的量。 NADH的量通过340nm处增加的吸光率来测量。

特异性,敏感度, 线性和准确性:

此方法特异性检测D-乳酸.

此方法特异性检测D-乳酸和L-乳酸。在D-乳酸锂（MW=96.0）的检测中，大约可以预期结果为96%（W/W），L-乳酸锂可以得到98%（W/W）。

检测的灵敏度为0.005 个吸光度单位。相当于样品最大体积为1.50 mL 时，样品浓度为0.107 mg/L(或者 1.60 mg/L ，样品体积0.1 mL) 。检测限为 0.214 mg/L，这是在0.010的吸光度差异，样品最大体积为1.50 mL为得到的。

D-乳酸检测的线性变化为0.5 到 30 μg 。在重复测定中，同一份样品溶液，可能产生0.005 到 0.010 个吸光光度值的差异。这就相当于样品1.50 mL，样品溶液中D-乳酸的浓度为0.107 到 0.214 mg/L 。如果样品在制备阶段被稀释过,结果要乘以稀释倍数F。如果样品制备阶段，称量过样品，比如10 g/L，可能会产生0.02 到 0.05 g/100 g 的差异。

干扰:

如果D-乳酸的转换在实验指定的时间内（大约5分钟）彻底完成，通常认为是没有发生任何干扰。不过，这个也可以进一步验证，通过在实验的完成时，向比色皿中加入D-乳酸混合物（大约每0.1mL15ug），吸光光度值有会明显增加。

样品中的干扰物质的分析可以通过加入一个内参来确定。需要进行一个标准品定量回收实验，通过这个回收实验（也就是在最初的提取步骤中向样品中加入D-乳酸）可以算出样品在处理和提取过程中的损失量。

D-Lactic Acid (D-Lactate) (Rapid)
For the rapid and specific assay of D-lactic acid in wine, beer, juice, milk, cheese, vinegar and meat products.

Catalogue Number: K-DATE
Content: 50 assays per kit

Appearance Five glass vials.
Specific Gravity not applicable
Solubility in Water Readily soluble.
pH Value 7.0
Odour none
Form powders and liquids
Stability stable in a freezer for five or more years
Ingredients Name CAS Proportion
Glycylglycine buffer 1 vial (glass)
Nicotinamide adenine dinucleotide 1 vial (glass)
D-Glutamate pyruvate transaminase enzyme 1 vial (glass)
D-Lactate Dehydrogenase enzyme 1 vial (glass)
D-Lactic acid standard solution 1 vial (glass)

K-DATE D-乳酸快速检测试剂盒
乙醛检测试剂盒K-ACHYD13
乙酸 (ACS;分析仪格式)检测试剂盒K-ACETAF13
乙酸(ACS;手工格式)检测试剂盒K-ACET14
乙酸(AK;分析仪格式)检测试剂盒K-ACETAK14
乙酸(RM;快速手工格式)检测试剂盒K-ACETRM15
α-淀粉酶(“Ceralpha”方法)检测试剂盒K-CERA15
氨检测试剂盒K-AMIA16
氨(快速)检测试剂盒K-AMIAR16
直链淀粉 /支链淀粉检测试剂盒K-AMYL17
阿拉伯糖检测试剂盒K-ARAB17
L-阿拉伯糖/ D-半乳糖(快速)检测试剂盒K-ARGA18
阿拉伯木聚糖检测试剂盒K-ARAXYL18
L-精氨酸 /尿素 /氨(快速)检测试剂盒K-LARGE19
L-抗坏血酸检测试剂盒K-ASCO19
L-天冬酰胺酸/ L-谷氨酸酰胺/氨(快速)检测试剂盒K-ASNAM20
阿斯巴甜检测试剂盒K-ASPTM20
可用碳水化合物 /膳食纤维检测试剂盒K-ACHDF21
?-淀粉酶(“Betamyl”方法)检测试剂盒K-BETA21
β-(Mixedlinkage)检测试剂盒K-BGLU22
β-检测试剂盒K-YBGL22
β-葡聚糖酶(麦芽和微生物)检测试剂盒K-MBGL23
K-DATE D-乳酸快速检测试剂盒
胆固醇检测试剂盒K-CHOL23
柠檬酸检测试剂盒K-CITR24
乙醇检测试剂盒K-ETOH24
蚁酸检测试剂盒K-FORM25
果糖(己糖激酶格式)检测试剂盒K-FRUCHK25
果糖(PAHBAH格式)检测试剂盒K-FRUC26
D-果糖 / D-葡萄糖检测试剂盒K-FRUGL26
D-果糖 / D-葡萄糖 (MegaQuant格式)检测试剂盒K-FRGLMQ27
半乳甘露聚糖检测试剂盒K-GALM27
葡甘露聚糖检测试剂盒K-GLUM28
D-葡萄糖酸 / D-葡萄糖酸内酯检测试剂盒K-GATE28
D-葡糖胺检测试剂盒K-GAME29
D-葡萄糖 (GOPOD格式)检测试剂盒K-GLUC29
D-葡萄糖(己糖激酶格式)检测试剂盒K-GLUHKR/L30
葡糖氧化酶检测试剂盒K-GLOX30
L-谷氨酰胺 /氨(快速)检测试剂盒K-GLNAM31

L-谷氨酸 (MSG)检测试剂盒K-GLUT31
甘油检测试剂盒K-GCROL32
D-3-羟丁酸检测试剂盒K-HDBA32
D-异柠檬酸检测试剂盒K-ISOC33
D-乳酸检测试剂盒K-DATE33
D-/L-乳酸检测试剂盒K-DLATE34
K-DATE D-乳酸快速检测试剂盒
L-乳酸检测试剂盒K-LATE34
乳糖 / D-半乳糖(快速)检测试剂盒K-LACGAR35
乳糖/蔗糖 / D-半乳糖检测试剂盒K-LACSU35
乳果糖检测试剂盒K-LACTUL36
D-苹果酸检测试剂盒K-DMAL36
L-苹果酸检测试剂盒K-LMALR/L37
L-苹果酸 (Analyser格式)检测试剂盒K-LMALAF37
L-苹果酸(MegaQuant格式)检测试剂盒K-LMALMQ38
麦芽糖/蔗糖/ D-葡萄糖检测试剂盒K-MASUG38
D-甘露醇 / L-阿拉伯糖醇检测试剂盒K-MANOL39
D-甘露糖 / D-果糖 / D-葡萄糖检测试剂盒K-MANGL39
果胶鉴定检测试剂盒K-PECID40
植酸检测试剂盒K-PHYT40
Primary氨基氮 (NOPA)检测试剂盒K-PANOPA41
丙酮酸检测试剂盒K-PYRUV41
棉籽糖/ D-半乳糖检测试剂盒K-RAFGA42
棉籽糖 /蔗糖 / D-葡萄糖检测试剂盒K-RAFGL42
抗性淀粉检测试剂盒K-RSTAR43
D-山梨醇 /木糖醇检测试剂盒K-SORB43
破损淀粉检测试剂盒K-SDAM44
琥珀酸检测试剂盒K-SUCC44
蔗糖 / D-果糖 / D-葡萄糖检测试剂盒K-SUFRG45
K-DATE D-乳酸快速检测试剂盒
蔗糖 / D-葡萄糖 (GOPOD格式)检测试剂盒K-SUCGL45
总膳食纤维检测试剂盒K-TDFR46
总淀粉 (GOPOD格式)检测试剂盒K-TSTA46
总淀粉(己糖激酶格式)检测试剂盒K-TSTAHK47
海藻糖检测试剂盒K-TREH47
尿素/氨(快速)检测试剂盒K-URAMR48
木聚糖酶 (Azo-Wax格式)检测试剂盒K-AZOWAX48
木聚糖酶(木聚糖酶 AX格式)检测试剂盒K-XYLS49
D-木糖检测试剂盒K-XYLOSE49

L-苹果酸检测试剂盒

L-苹果酸检测试剂盒L-Malic Acid Assay Kit (Manual Format)

产品货号： K-LMALL

产品品名： L- 苹果酸检测试剂盒（大）

英文品名： L-Malic Acid Assay Kit (Large)

规格型号： 116 assays

原理：

反应时间： 3min

检测限： 0.25 mg/L,

适用样品： 饮料和食品以及其他原料

优点：

l 成本低

l 使用期间所有试剂的稳定性 >2 年

l 操作简单

l 包含标准品

REFERENCES:

Mollering, H. (1985). L-Malate. In Methods of Enzymatic Analysis (Bergmeyer, H. U., ed.), 3rd ed., Vol. VII, pp. 39-47, VCH Publishers (UK) Ltd., Cambridge, UK.
AOAC Official Methods of Analysis (2002). Method 993.05 “L-Malic acid/total malic acid ratio in apple juice”. 17th ed. Chapter 37, p. 15.
Gorin, N. (1976). Differences in L-malate determined enzymatically or titrimetrically in golden delicious apples. Zeitschrift fur Lebensmittel-Unterschung und-Forschung, 162, 259-261.
Klopper, W. J., Angelino, S. A. G. F., Tuning, B. & Vermeire, H. A. (1986). Organic acids and glycerol in beer. J. Inst. Brew. 92, 225-228.
Elkins, E. R. & Freund, W. (1994). Detection of adulteration in apple juice by L-malic/total malic acid ratio: Collaborative Study. J. AOAC Int . 77, 411-415.

商品说明：

L-Malic Acid (Regular) Assay Kit, for the specific assay of L-malic acid (L-malate) in beverages and food products.

Manual format UV-method for the determination of L-Malic Acid in foodstuffs, beverages and other materials

Principle:
(L-malate dehydrogenase)
(1) L-Malic acid + NAD⁺ ↔ oxaloacetate + NADH + H⁺

(glutamate-oxaloacetate transaminase)
(2) Oxaloacetate + L-glutamate → L-aspartate + 2-oxoglutarate

Kit size: (K-LMALR)
58 assays (manual) / 580 (microplate) or
(K-LMALL)
116 assays (manual) / 1160 (microplate)
Method: Spectrophotometric at 340 nm
Reaction time: ~ 3 min
Detection limit: 0.25 mg/L
Application examples:
Wine, beer, fruit juices, soft drinks, candies, fruit and vegetables,
bread, cosmetics, pharmaceuticals and other materials (e.g. biological
cultures, samples, etc.)
Method recognition:
Methods based on this principle have been accepted by AOAC, EEC,
EN, NF, NEN, DIN, GOST, OIV, IFU, AIJN and MEBAK

Advantages

PVP incorporated to prevent tannin inhibition
Both enzymes supplied as stable suspensions
Very competitive price (cost per test)
All reagents stable for > 2 years after preparation
Very rapid reaction (~ 3 min)
Mega-Calc™ software tool is available from our website for hassle-free raw data processing
Standard included
Extended cofactors stability
Suitable for manual and microplate format

乳果糖检测试剂盒 Lactulose Assay Kit

产品货号：K-LACTUL

品牌：Megazyme

产品品名：乳果糖检测试剂盒

英文品名：Lactulose Assay Kit

规格型号：50 assays

商品说明：

乳果糖快速检测方法—Megazyme乳果糖快速检测试剂盒

Megazyme乳果糖检测试剂盒采用酶法分析原理，可定量检测鲜奶、UHT奶、浓缩乳和奶粉等各种乳品中的乳果糖。

与农业部新标准采用相同的检测原理和方法

该试剂盒采用ISO方法11285：2004，经过改进后，更加快速和灵敏

灵敏度是传统己糖激酶法的两倍

成本低

试剂配制后可稳定保存2年

采用试剂盒形式，包含检测必需的所有酶

提供计算软件，使数据处理更方便

包含标准品

The Lactulose Assay Kit is suitable for the specific, rapid and sensitive measurement and analysis of lactulose in milk and milk-based samples. Reagents included in this kit may also be prepared for use in the procedure described by ISO Method 11285:2004.

UV-method for the determination of Lactulose in milk and
foodstuffs containing dairy products

Principle:
(β-galactosidase)
(1) Lactulose + H₂O → D-galactose + D-fructose

(glucose oxidase + catalase + H₂O₂)
(2) D-Glucose + H₂O + O₂ → D-gluconic acid + H₂O₂

(hexokinase)
(3) D-Fructose + ATP → F-6-P + ADP

(phosphoglucose isomerase)
(4) F-6-P → G-6-P

(glucose-6-phosphate dehydrogenase)
(5) G-6-P + NADP⁺ → gluconate-6-phosphate + NADPH + H⁺

(gluconate-6-phosphate dehydrogenase)
(6) Gluconate-6-phosphate + NADP⁺ → ribulose-5-phosphate + NADPH
+ CO₂ + H⁺

Kit size: 50 assays
Method: Spectrophotometric at 340 nm
Reaction time: ~ 120 min
Detection limit: 4.8 mg/L
Application examples:
Milk, dairy products and foods containing milk
Method recognition: Novel method

Advantages

Twice the sensitivity of traditional hexokinase based lactulose methods
Very cost effective
All reagents stable for > 2 years after preparation
Mega-Calc™ software tool is available from our website for hassle-free raw data processing
Standard included

甘油检测试剂盒 Glycerol GK Assay Kit

产品货号：K-GCROL

产品品名：甘油检测试剂盒

英文品名：Glycerol Assay Kit

规格型号： 70 assays per kit

甘油是食品加工业中通常使用的甜味剂和保湿剂，大多出现在运动食品和代乳品中。

上海金畔生物提供甘油检测试剂盒，紫外吸光度法（ 340nm ）测定食品、饮料及其他原料中的甘油。

原理：

反应时间： 5min

检测限： 0.37mg/L

适用样品： 葡萄酒（葡萄汁）、啤酒、烈酒、醋、杏仁蛋白软糖、果汁、软饮料、牙膏、蜂蜜、烟、纸（硬纸板）、化妆品

The Glycerol test kit is a simple, reliable, rapid and accurate method for the measurement and analysis of Glycerol in beverages, foodstuffs and other materials.
Suitable for manual and microplate formats.

UV-method for the determination of Glycerol in foodstuffs,
beverages and other materials

Principle:
(glycerokinase)
(1) Glycerol + ATP → L-glycerol-3-phosphate + ADP

(pyruvate kinase)
(2) ADP + PEP → ATP + pyruvate

(L-lactate dehydrogenase)
(3) Pyruvate + NADH + H⁺ → L-lactic acid + NAD⁺

Kit size: 70 assays (manual) / 700 (microplate)
Method: Spectrophotometric at 340 nm
Reaction time: ~ 5 min
Detection limit: 0.34 mg/L
Application examples:
Wine (and grape juice), beer, spirits, vinegar, marzipan, fruit juices,
soft drinks, toothpaste, honey, tobacco, paper (and cardboard),
cosmetics, pharmaceuticals, soap and other materials (e.g. biological
cultures, samples, etc.)
Method recognition:
Methods based on this principle have been accepted by OIV and
MEBAK

Advantages

Novel tablet format for increased stability
Very competitive price (cost per test)
All reagents stable for > 2 years as supplied
Very rapid reaction
Mega-Calc™ software tool is available from our website for hassle-free raw data processing
Standard included
Suitable for manual and microplate formats

优点：

l 新型的片剂形式增加了稳定性

l 价格低廉（每次检测成本）

l 所有试剂配制后的稳定性 >2 年

l 反应快

l 该方法已通过 OIV ， MEBAK 以及德国和瑞士的认证

商品说明：

The Glycerol GK test kit is a simple, reliable and accurate method for the measurement and analysis of glycerol in beverages, foodstuffs and other material. Based on use of ADP-glucokinase and increase in absorbance on conversion of NAD+ to NADH.
Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.
Suitable for manual, auto-analyser and microplate formats.

UV-method for the determination of Glycerol in foodstuffs,
beverages and other materials

Principle:
(glycerokinase)
(1) Glycerol + ATP → L-glycerol-3-phosphate + ADP

(ADP-GK)
(2) ADP + D-glucose → G-6-P + AMP

(glucose-6-phosphate dehydrogenase)
(3) G-6-P + NAD⁺ → gluconate-6-phosphate + NADH + H⁺

Kit size: 70 assays (manual) / 700 (microplate)
/ 600 (auto-analyser)
Method: Spectrophotometric at 340 nm
Reaction time: ~ 7 min
Detection limit: 0.37 mg/L
Application examples:
Wine (and grape juice), beer, spirits, vinegar, marzipan, fruit juices,
soft drinks, toothpaste, honey, tobacco, paper (and cardboard),
cosmetics, pharmaceuticals, soap and other materials (e.g. biological
cultures, samples, etc.)
Method recognition: Novel method

Advantages

Novel tablet format for increased stability
Very competitive price (cost per test)
All reagents stable for > 2 years as supplied
Very rapid reaction
Positive reaction (assay proceeds with an increase in absorbance)
Mega-Calc™ software tool is available from our website for hassle-free raw data processing
Standard included
Suitable for manual, microplate and auto-analyser formats

D-果糖/D-葡萄糖检测试剂盒 D-Fructose/D-Glucose Assay Kit

1) 英文：D-Fructose/D-Glucose Assay Kit;

2) 中文：D-果糖/D-葡萄糖检测试剂盒;

3) 规格: 110; 4) 货号:K-FRUGL

方法认证：AOAC，EN。NEN。NF。DIN，GOST，OIV，IFU ,AIJN,MEBAK,IOCCC以及德国、瑞士、奥地利和意大利的认证

优点：加入PVP防止丹宁酸的抑制；适用于手工和自动分析仪检测（优良的机器稳定性）；已通过法国叙兹拉鲁塞葡萄酒大学的验证；价格低廉（每次检测成本）；所有试剂配制后的稳定性〉2年（适用于手工分析）；反应快25℃或37℃；包含稳定的D-葡萄糖/D-果糖标准溶液

检测原理：

检测方法：紫外风光光度法

检测波长：340nm；

反应时间：13min；

检测限：066mg/L；

适用样品：葡萄酒、啤酒、果汁、软饮料、牛奶、果酱、蜂蜜、食疗食品、烤食品、糖果、甜点、糖食、冰淇淋、水果和蔬菜、调味品、烟、化妆品、医药品、纸及其他原料（如生物培养基等样品）；

商品说明：

D-Fructose/D-Glucose test kit, an enzymatic UV-method for the measurement and analysis of D-fructose and/or D-glucose in plant and food products.
Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.
Suitable for manual, auto-analyser and microplate formats.

维生素C[L-抗坏血酸]检测试剂盒

产品货号：K-ASCO

品牌：Megazyme

产品品名：维生素C（L-抗坏血酸）检测试剂盒

英文品名：Ascorbic Acid (L-Ascorbate) Assay Kit

规格型号：40 assays (manual) / 400 assays (microplate) / 400 assays (auto-analyser)

商品说明：

For the specific assay of L-ascorbic acid in beverages, meat, flour, dairy and vegetable products. Content:40 assays per kit

Colourimetric method for the determination of L-Ascorbic Acid
in foodstuffs, feed, wine and other materials

Principle:
(5-methylphenazinium methosulphate)
(1) L-Ascorbic acid + R-H₂ + MTT → dehydroascorbate + MTT-formazan + H⁺
(ascorbic acid oxidase)
(2) L-Ascorbic acid + ½O₂ → dehydroascorbate + H₂O

Kit size:                             40 assays (manual) / 400 (microplate)
/ 400 (auto-analyser)
Method:                             Spectrophotometric at 578 nm
Reaction time:                   ~ 8 min
Detection limit:               0.175 mg/L
Application examples:
Wine, beer, fruit juices, soft drinks, jam, milk, dairy products
(e.g. cheese), dietetic foods, baby foods, processed meat, baking
additives, fruit and vegetables (e.g. tomato and potato),
pharmaceuticals, feed and other materials (e.g. biological cultures,
samples, etc.)
Method recognition:
Methods based on this principle have been accepted by MEBAK

Advantages

Very competitive price (cost per test)
All reagents stable for > 6 months after preparation
Mega-Calc™ software tool is available from our website for hassle-free raw data processing
Standard included
Suitable for manual, microplate and auto-analyser formats

麦芽淀粉酶检测试剂盒 Malt Amylase Assay Kit

货号： K-MALTA

品名：麦芽淀粉酶检测试剂盒 Malt Amylase Assay Kit

型号：100 assays per kit

α-淀粉酶，β-淀粉酶在淀粉降解代谢过程中或发酵糖发芽或谷物麦芽中发挥重要作用。

上海子起生物提供麦芽淀粉酶检测试剂盒，比色法（400nm）测定谷物、麦芽、食品、饮料和发酵产品中的α-淀粉酶和Beta-淀粉酶。

原理：（说明书请致电 400-811-9081）

(1) α-淀粉酶检测利用“Ceralpha” 方法

(2) Beta-淀粉酶检测利用 “Betamyl-3” 方法

反应时间：

麦芽淀粉酶检测试剂盒 K-MALTA
~ 20 min (Ceralpha 方法)

~ 10 min (Betamyl-3 方法)

检测限：0.05 U/mL

适用样品：面粉、麦芽、发酵液和其他原料

优点：

价格低廉（每次检测成本）

所有试剂配制后的稳定性>2年

只适用于酶法试剂盒

特异性高

方法简单

包含标准品

方法认证：AOAC (Method 2002.01), AACC (Method 22-02.01),ICC (Standard No. 303), RACI (Standard Method), and CCFRA (Flour Testing Working Group Method 0018)

RACI (标准方法)

淀粉损失检测试剂盒

淀粉损失检测试剂盒，Starch Damage Assay Kit

英文名：Starch Damage Assay Kit

中文品名：淀粉损伤检测试剂盒

货号：K-SDAM

规格：200 assays per kit，200 次检测

应用：淀粉损伤检测试剂盒，用于谷物面粉中淀粉损伤的检测和分析。

原理：

谷物淀粉中淀粉损伤紫外检测方法

检测方法：分光光度法 @510 nm

反应时间：~ 40分钟

检测限：样品重量的0.5-100%

应用案例：

谷物面粉和其它食材。

方法识别：

符合AACC (方法 76-31.01), ICC (标准号 164) 和 RACI (标准方法)。

试剂盒组成：

Bottle 1:	真菌α-淀粉酶(pH5.4和40°C条件下，10 mL, 1,000 U/mL on Ceralpha reagent* )。硫酸铵悬浮液。
	4°C下稳定超过 3年
Bottle 2:	淀粉葡萄糖苷酶(pH 4.5 和40°C条件下，4 mL, 200 U/mL 在可溶性淀粉中)。硫酸铵悬浮液。
	4°C稳定超过3年
Bottle 3:	GOPOD 试剂缓冲液。缓冲液(50 mL, pH7.4), 对羟基苯甲酸和叠氮钠(0.095% w/v).
	4°C稳定超过4年
Bottle 4:	GOPOD 试剂酶。葡萄糖氧化酶，过氧化酶和4-氨基安替比林。冻干粉。
	-20°C稳定超过5年
Bottle 5:	D-葡萄糖标准溶液(5 mL, 1.5 mg/mL) 溶于0.2% (w/v)苯甲酸。
	室温下稳定超过5年
Bottle 6:	小麦面粉标样。淀粉损伤程度在小瓶标签上注明。
	室温下稳定超过5年

Megazyme 淀粉损伤检测试剂盒（K-SDAM）

淀粉损伤检测试剂盒, Starch Damage Assay Kit , 货号：K-SDAM

品牌：Megazyme

货号：K-SDAM

中文品名：淀粉损伤检测试剂盒

品名：Starch Damage Assay Kit

规格：200 次检测

应用：淀粉损伤检测试剂盒，用于谷物面粉中淀粉损伤的检测和分析。

原理：

谷物淀粉中淀粉损伤紫外检测方法

检测方法：分光光度法 @510 nm

反应时间：~ 40分钟

检测限：样品重量的0.5-100%

应用案例：

谷物面粉和其它食材。

方法识别：

符合AACC (方法 76-31.01), ICC (标准号 164) 和 RACI (标准方法)。

试剂盒组成：

Bottle 1:	真菌α-淀粉酶(pH5.4和40°C条件下，10 mL, 1,000 U/mL on Ceralpha reagent* )。硫酸铵悬浮液。
	4°C下稳定超过 3年
Bottle 2:	淀粉葡萄糖苷酶(pH 4.5 和40°C条件下，4 mL, 200 U/mL 在可溶性淀粉中)。硫酸铵悬浮液。
	4°C稳定超过3年
Bottle 3:	GOPOD 试剂缓冲液。缓冲液(50 mL, pH7.4), 对羟基苯甲酸和叠氮钠(0.095% w/v).
	4°C稳定超过4年
Bottle 4:	GOPOD 试剂酶。葡萄糖氧化酶，过氧化酶和4-氨基安替比林。冻干粉。
	-20°C稳定超过5年
Bottle 5:	D-葡萄糖标准溶液(5 mL, 1.5 mg/mL) 溶于0.2% (w/v)苯甲酸。
	室温下稳定超过5年
Bottle 6:	小麦面粉标样。淀粉损伤程度在小瓶标签上注明。
	室温下稳定超过5年

优点：

价格非常具有竞争力
所有试剂制备后可以稳定保存超过2年
仅提供酶法检测试剂盒
特异性
操作简单
官网提供Mega-Calc™ 软件工具用于一站式原始数据处理
包含标准品

使用说明书：https://secure.megazyme.com/files/Booklet/K-SDAM_DATA.pdf

The Starch Damage test kit is suitable for the measurement and analysis of starch damage in cereal flours.

Colourimetric method for the determination of Starch Damage
in cereal flours

Principle:
(fungal α-amylase)
(1) Damaged (or gelatinised) starch + H₂O → maltodextrins

(amyloglucosidase)
(2) Maltodextrins + H₂O → D-glucose

(glucose oxidase)
(3) D-Glucose + H₂O + O₂ → D-gluconate + H₂O₂

(peroxidase)
(4) 2H₂O₂ + p-hydroxybenzoic acid + 4-aminoantipyrine →
quinoneimine + 4H₂O

Kit size: 200 assays
Method: Spectrophotometric at 510 nm
Total assay time: ~ 40 min
Detection limit: 0.5-100% of sample weight
Application examples:
Cereal flours and other materials
Method recognition:
AACC (Method 76-31.01), ICC (Standard No. 164), and RACI (Standard
Method)

Advantages

Very cost effective
All reagents stable for > 2 years after preparation
Only enzymatic kit available
Very specific
Simple format
Mega-Calc™ software tool is available from our website for hassle-free raw data processing
Standard included

参考文献：

An improved enzymic method for the measurement of starch damage in wheat flour. Gibson, T. S., Al Qalla, H. & McCleary, B. V. (1992). Journal of Cereal Science, 15(1), 15-27.

Collaborative evaluation of an enzymatic starch damage assay kit and comparison with other methods. Gibson, T. S., Kaldor, C. J. & McCleary, B. V. (1993). Cereal Chem., 70(1), 47-51.

Measurement of total starch in cereal products by amyloglucosidase-alpha-amylase method: collaborative study. McCleary, B. V., Gibson, T. S. & Mugford, D. C. (1997). Journal of AOAC International, 80, 571-579.

Measurement of carbohydrates in grain, feed and food. McCleary, B. V., Charnock, S. J., Rossiter, P. C., O’Shea, M. F., Power, A. M. & Lloyd, R. M. (2006). Journal of the Science of Food and Agriculture, 86(11), 1648-1661.

Starch properties, in vitro digestibility and sensory evaluation of fresh egg pasta produced from oat, teff and wheat flour. Hager, A. S., Czerny, M., Bez, J., Zannini, E. & Arendt, E. K. (2013). Journal of Cereal Science, 58(1), 156-163.

Effect of sorghum flour composition and particle size on quality properties of gluten-free bread. Trappey, E. F., Khouryieh, H., Aramouni, F. & Herald, T. (2014). Food Science and Technology International, 1082013214523632.

Nutritional properties and ultra-structure of commercial gluten free flours from different botanical sources compared to wheat flours. Hager, A. S., Wolter, A., Jacob, F., Zannini, E. & Arendt, E. K. (2012). Journal of Cereal Science, 56(2), 239-247.

Quality variations in flours used for pretzel manufacturing. Yao, N. & Seetharaman, K. (2010). International Journal of Food Science & Technology, 45(10), 2052-2061.

Effect of corn preparation methods on dry-grind ethanol production by granular starch hydrolysis and partitioning of spent beer solids. Lamsal, B. P., Wang, H. & Johnson, L. A. (2011). Bioresource Technology, 102(12), 6680-6686.

Flaking as a corn preparation technique for dry-grind ethanol production using raw starch hydrolysis. Lamsal, B. P. & Johnson, L. A. (2012). Journal of Cereal Science, 56(2), 253-259.

Chemical composition and functional properties of native chestnut starch (Castanea sativa Mill). Cruz, B. R., Abraão, A. S., Lemos, A. M. & Nunes, F. M. (2013). Carbohydrate Polymers, 94(1), 594-602.

Changes in rice with variable temperature parboiling: thermal and spectroscopic assessment. Himmelsbach, D. S., Manful, J. T. & Coker, R. D. (2008). Cereal chemistry, 85(3), 384-390.

Determination of formulation and processing factors affecting slowly digestible starch, protein digestibility and antioxidant capacity of extruded sorghum–maize composite flour. Licata, R., Chu, J., Wang, S., Coorey, R., James, A., Zhao, Y. & Johnson, S. (2014). International Journal of Food Science & Technology, 49(5), 1408-1419.

Analysis of starch amylolysis using plots for first-order kinetics. Butterworth, P. J., Warren, F. J., Grassby, T., Patel, H. & Ellis, P. R. (2012). Carbohydrate Polymers, 87(3), 2189-2197.